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BMI1 Monoclonal antibody

BMI1 Monoclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, monkey

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2E10A11

Cat no : 66161-1-Ig

Synonyms

BMI1, PCGF4, Polycomb complex protein BMI 1, RING finger protein 51, RNF51



Tested Applications

Positive WB detected inPC-12 cells, HT-1080 cells, U2OS cells, U-937 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOS-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

66161-1-Ig targets BMI1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BMI1 fusion protein Ag21284
Full Name BMI1 polycomb ring finger oncogene
Calculated molecular weight 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC011652
Gene symbol BMI1
Gene ID (NCBI) 648
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.

Protocols

Product Specific Protocols
WB protocol for BMI1 antibody 66161-1-IgDownload protocol
IHC protocol for BMI1 antibody 66161-1-IgDownload protocol
IF protocol for BMI1 antibody 66161-1-IgDownload protocol
IP protocol for BMI1 antibody 66161-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Aging (Albany NY)

Identification of a mesenchymal-related signature associated with clinical prognosis in glioma.

Authors - Zhengwei Zhang
humanWB,IF

Cancer Sci

Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.

Authors - Guan-Zhong Qiu
human,mouseWB,IHC

Mol Cancer Ther

Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.

Authors - Anindya Dey
humanWB

Autophagy

Inhibition of BMI1 induces autophagy-mediated necroptosis.

Authors - Anindya Dey
  • KD Validated