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- KD/KO Validated
BMI1 Monoclonal antibody
BMI1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 66161-1-Ig
Validation Data Gallery
|Positive WB detected in
|PC-12 cells, HT-1080 cells, U2OS cells, U-937 cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:10-1:100
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66161-1-Ig targets BMI1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, monkey samples.
|human, mouse, monkey
|Host / Isotype
|Mouse / IgG2b
|BMI1 fusion protein Ag21284
|BMI1 polycomb ring finger oncogene
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.
|Product Specific Protocols
|WB protocol for BMI1 antibody 66161-1-Ig
|IHC protocol for BMI1 antibody 66161-1-Ig
|IF protocol for BMI1 antibody 66161-1-Ig
|IP protocol for BMI1 antibody 66161-1-Ig
|Click here to view our Standard Protocols
Inhibition of BMI1 induces autophagy-mediated necroptosis.
Clin Transl Med
Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.
J Bone Miner Res
Bmi-1 Overexpression Improves Sarcopenia Induced by 1,25(OH)2 D3 Deficiency and Downregulates GATA4-Dependent Rela Transcription
Aging (Albany NY)
Identification of a mesenchymal-related signature associated with clinical prognosis in glioma.
Mol Cancer Ther
Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.
Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.