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BMI1 Monoclonal antibody
BMI1 Monoclonal Antibody for IF, IHC, IP, WB, ELISA
Cat no : 66161-1-Ig
Validation Data Gallery
|Positive WB detected in||PC-12 cells, HT-1080 cells, U2OS cells, U-937 cells|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue, human lymphoma tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||COS-7 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
66161-1-Ig targets BMI1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, monkey samples.
|Tested Reactivity||human, mouse, monkey|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||BMI1 fusion protein Ag21284|
|Full Name||BMI1 polycomb ring finger oncogene|
|Calculated molecular weight||37 kDa|
|Observed molecular weight||37-43 kDa|
|GenBank accession number||BC011652|
|Gene ID (NCBI)||648|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.
|Product Specific Protocols|
|WB protocol for BMI1 antibody 66161-1-Ig||Download protocol|
|IHC protocol for BMI1 antibody 66161-1-Ig||Download protocol|
|IF protocol for BMI1 antibody 66161-1-Ig||Download protocol|
|IP protocol for BMI1 antibody 66161-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Inhibition of BMI1 induces autophagy-mediated necroptosis.
Clin Transl Med
Bmi-1-RING1B prevents GATA4-dependent senescence-associated pathological cardiac hypertrophy by promoting autophagic degradation of GATA4.
Mol Cancer Ther
Evaluating the Mechanism and Therapeutic Potential of PTC-028, a Novel Inhibitor of BMI-1 Function in Ovarian Cancer.
Ubiquitin-specific protease 22 acts as an oncoprotein to maintain glioma malignancy through deubiquitinating B cell-specific Moloney murine leukemia virus integration site 1 for stabilization.
Exp Cell Res
EPS8 supports pancreatic cancer growth by inhibiting BMI1 mediated proteasomal degradation of ALDH7A1.
Stem Cells Dev
Chronic alcohol reduces bone mass through inhibiting proliferation and promoting aging of endothelial cells in type-H vessels.