BMP2 Monoclonal antibody

• 

  WB analysis of pig bone marrow using 66383-1-Ig

  pig bone marrow tissue were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66383-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66383-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Caco-2 using 66383-1-Ig

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 66383-1-Ig (BMP2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human small intestine using 66383-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human small intestine using 66383-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66383-1-Ig (BMP2 Antibody) at dilution of 1:200 (under 40x lens).

BMP2 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66383-1-Ig

Clone No.1C5C2

Publications(70)

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF, ELISA

Synonyms

BMP 2, BMP2A, BMP-2, BMP-2A, BMP 2A

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Tested Applications

• Positive WB detected in: pig bone marrow tissue, Caco-2 cells, HeLa cells, pig aorta tissue
• Positive IHC detected in: human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 59 publications below
• IHC: See 8 publications below
• IF: See 7 publications below

Product Information

66383-1-Ig targets BMP2 in WB, IHC, IF, ELISA applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13501

  Product name: Recombinant human BMP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-396 aa of BC069214

  Sequence: MVAGTRCLLALLLPQVLLGGAAGLVPELGRRKFAAASSGRPSSQPSDEVLSEFELRLLSMFGLKQRPTPSRDAVVPPYMLDLYRRHSGQPGSPAPDHRLERAASRANTVRSFHHEESLEELPETSGKTTRRFFFNLSSIPTEEFITSAELQVFREQMQDALGNNSSFHHRINIYEIIKPATANSKFPVTSLLDTRLVNQNASRWESFDVTPAVMRWTAQGHANHGFVVEVAHLEEKQGVSKRHVRISRSLHQDEHSWSQIRPLLVTFGHDGKGHPLHKREKRQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR

• Full Name: bone morphogenetic protein 2
• Calculated Molecular Weight: 396 aa, 44 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC069214
• Gene Symbol: BMP2
• Gene ID (NCBI): 650
• RRID: AB_2881759
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P12643
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer; 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. Two band isofroms of BMP2 are present (PMID: 35846837).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Theranostics; Multifunctionally disordered TiO2 nanoneedles prevent periprosthetic infection and enhance osteointegration by killing bacteria and modulating the osteoimmune microenvironment; Authors - Yangmengfan Chen
human	WB	ACS Appl Mater Interfaces; Nitrogen-Doped Multiwalled Carbon Nanotubes Enhance Bone Remodeling through Immunomodulatory Functions.; Authors - Haifang Li
human	WB	Angiogenesis; Linalool inhibits the angiogenic activity of endothelial cells by downregulating intracellular ATP levels and activating TRPM8.; Authors - Vivien Becker
	WB	Cell Prolif; Effects of tetrahedral DNA nanostructures on the treatment of osteoporosis; Authors - Weitong Cui
human	WB	Br J Pharmacol; Andrographolide regulates H3 histone lactylation by interfering with p300 to alleviate aortic valve calcification; Authors - Chunli Wang
	WB	Phytother Res; Bavachinin selectively modulates PPAR γ and maintains bone homeostasis in Type 2 Diabetes; Authors - Jingwen Liu