BRAF Polyclonal antibody

BRAF Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20899-1-AP

Synonyms

B RAF1, BRAF, BRAF1, p94, Proto oncogene B Raf, RAFB1



Tested Applications

Positive WB detected inNIH/3T3 cells, A431 cells, A375 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman malignant melanoma tissue, human thyroid cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 20899-1-AP is BRAF Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRAF fusion protein Ag15014
Full Name v-raf murine sarcoma viral oncogene homolog B1
Calculated molecular weight 766 aa, 84 kDa
GenBank accession numberBC101757
Gene symbol BRAF
Gene ID (NCBI) 673
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

B-Raf proto-oncogene serine/threonine kinase(BRAF), is a protein belonging to the raf/mil family of serine/threonine protein kinases. BRAF plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. BRAF is associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. BRAF is also associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. BRAF plays a role in the PD-1/PDL-1 pathway. In some cancers, BRAF is activated by rearrangements that fuse its kinase domain to 5' partner genes and then has different molecular weights (PMID: 23890088).

Protocols

Product Specific Protocols
WB protocol for BRAF antibody 20899-1-APDownload protocol
IHC protocol for BRAF antibody 20899-1-APDownload protocol
IF protocol for BRAF antibody 20899-1-APDownload protocol
IP protocol for BRAF antibody 20899-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Cancer

VPS33B negatively modulated by nicotine functions as a tumor suppressor in colorectal cancer.

Authors - Yiyu Chen
humanWB

Thorac Cancer

High expression of KITLG is a new hallmark activating the MAPK pathway in type A and AB thymoma.

Authors - Zhaoyu Yang