BRAWNIN Polyclonal antibody

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  WB analysis using 30212-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30212-1-AP (BRAWNIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of U-251 using 30212-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U-251 using 30212-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of A431 using 30212-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

BRAWNIN Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 30212-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

BR, BRAWNIN, C12orf73, DKFZp547P055, FLJ13975, UQCC6

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Tested Applications

• Positive WB detected in: K-562 cells, MCF-7 cells
• Positive IF/ICC detected in: U-251 cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:400-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30212-1-AP targets BRAWNIN in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRAWNIN fusion protein Ag32955
• Full Name: chromosome 12 open reading frame 73
• Calculated Molecular Weight: 8 kDa
• Observed Molecular Weight: 9 kDa
• Gene Symbol: C12orf73
• Gene ID (NCBI): 728568
• RRID: AB_3086265
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q69YU5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ubiquinol-cytochrome-c reductase complex assembly factor 6 (UQCC6) is also named as BRAWNIN, BR and C12orf73, and belongs to the UQCC6 family. BRAWNIN (BR), a 71 a.a. peptide encoded by C12orf73, is essential for respiratory chain complex III (CIII) assembly. In human cells, BRAWNIN is induced by the energy-sensing AMPK pathway, and its depletion impairs mitochondrial ATP production. In zebrafish, Brawnin deletion causes complete CIII loss, resulting in severe growth retardation, lactic acidosis and early death (PMID:32161263).  BR is also detectable in human cardiac and skeletal muscle where it displays a staining pattern characteristic of the mitochondria network. BR protein abundance in mouse tissues correlated well with that of mitochondrial respiratory chain proteins, being high in brown adipose, cardiac and skeletal muscle but virtually undetectable in white adipose tissue (PMID:32161263). BR resides in the IMM and not in the outer mitochondrial membrane (OMM) (PMID:32161263).