BRAWNIN Polyclonal antibody, PBS Only

• 

  WB analysis using 30212-1-AP (same clone as 30212-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30212-1-AP (BRAWNIN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30212-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 30212-1-AP (same clone as 30212-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30212-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U-251 using 30212-1-AP (same clone as 30212-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30212-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 30212-1-AP (same clone as 30212-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using BRAWNIN antibody (30212-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 30212-1-PBS in a different storage buffer formulation.

BRAWNIN Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 30212-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

---

Product Information

30212-1-PBS targets BRAWNIN in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRAWNIN fusion protein Ag32955
• Full Name: chromosome 12 open reading frame 73
• Calculated Molecular Weight: 8 kDa
• Observed Molecular Weight: 9 kDa
• Gene Symbol: C12orf73
• Gene ID (NCBI): 728568
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q69YU5
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Ubiquinol-cytochrome-c reductase complex assembly factor 6 (UQCC6) is also named as BRAWNIN, BR and C12orf73, and belongs to the UQCC6 family. BRAWNIN (BR), a 71 a.a. peptide encoded by C12orf73, is essential for respiratory chain complex III (CIII) assembly. In human cells, BRAWNIN is induced by the energy-sensing AMPK pathway, and its depletion impairs mitochondrial ATP production. In zebrafish, Brawnin deletion causes complete CIII loss, resulting in severe growth retardation, lactic acidosis and early death (PMID:32161263).  BR is also detectable in human cardiac and skeletal muscle where it displays a staining pattern characteristic of the mitochondria network. BR protein abundance in mouse tissues correlated well with that of mitochondrial respiratory chain proteins, being high in brown adipose, cardiac and skeletal muscle but virtually undetectable in white adipose tissue (PMID:32161263). BR resides in the IMM and not in the outer mitochondrial membrane (OMM) (PMID:32161263).