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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using BUB3 Polyclonal antibody (10123-2-AP)

    IF Staining of HepG2 using 10123-2-AP

    Immunofluorescent analysis of HepG2 cells, using BUB3 antibody 10123-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10123-2-AP targets BUB3 in IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BUB3 fusion protein Ag0167 Predict reactive species
Full Name budding uninhibited by benzimidazoles 3 homolog (yeast)
Calculated Molecular Weight 37 kDa
GenBank Accession NumberBC005138
Gene Symbol BUB3
Gene ID (NCBI) 9184
RRIDAB_2066209
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43684
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BUB3 is a conserved component of the mitotic spindle assembly complex and is an essential compent of spindle-assembly checkpoint (SAC) signaling that operates during early embryogenesis. BUB3 null embryos treated with a spindle-depolymerising agent fail to arrest in metaphase and show an increase in mitotic disarray. In mitosis, the SAC prevents anaphase onset until all chromosomes have been attached to the spindle microtubules and aligned correctly at the equatorial metaphase plate. BUB3 is involved in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments in mammalian oocyte meiosis.

Protocols

Product Specific Protocols
IF protocol for BUB3 antibody 10123-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols