ATG14/Barkor (N-terminal) Antibody

• Featured Product
• KD/KO Validated

ATG14/Barkor (N-terminal) Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (1)

Cat no : 19491-1-AP

Synonyms

ATG14, Atg14L, Barkor, KIAA0831

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Validation Data Gallery

• 

  WB analysis of HEK-293 cells using 19491-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

• 

  IF staining of MCF-7 cells using 19491-1-AP

  Immunofluorescent analysis of ( -20°C Ethanol) fixed MCF-7 cells using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 12 publications below
• IF: See 2 publications below

Product Information

19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: bovine, dog, human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: KIAA0831
• Calculated molecular weight: 55 kDa
• Observed molecular weight: 65-68 kDa
• GenBank accession number: NM_014924
• Gene symbol: KIAA0831
• Gene ID (NCBI): 22863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).

Publications

Species	Application	Title
human	WB,IF	Mol Cell; Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy.; Authors - Jose Norberto S Vargas
mouse	IF	Mol Psychiatry; Sequential formation of different layers of dystrophic neurites in Alzheimer's brains.; Authors - Md Golam Sharoar
bovine	WB	J Microbiol; Bovine viral diarrhea virus infection induces autophagy in MDBK cells.; Authors - Qiang Fu ; KD Validated
human	WB	Cell Cycle; PMID: 31885310; Authors - Zhenhua Hu
human	WB	Onco Targets Ther; MicroRNA-375 Targets ATG14 to Inhibit Autophagy and Sensitize Hepatocellular Carcinoma Cells to Sorafenib.; Authors - Shuo Yang
dog	WB	J Gen Virol; Lentivirus-mediated bta-miR-29b overexpression interferes with bovine viral diarrhea virus replication and viral infection-related autophagy by directly targeting ATG14 and ATG9A in MDBK cells.; Authors - Qiang Fu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Charlotte (Verified Customer) (01-25-2018)

I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: U2OS

ATG14/Barkor (N-terminal) Antibody Immunofluorescence, validation (1:100 dilution) in U2OS (Cat no:19491-1-AP)

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