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ATG14/Barkor (N-terminal) Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 19491-1-AP

Featured Product KD/KO validated

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Con: 52 μg/150 μl

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Tested Applications
Applications:
ELISA, IF, IP, WB
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19491-1-AP;mouse brain tissue

mouse brain tissue were subjected to SDS PAGE followed by western blot with 19491-1-AP(ATG14/Barkor (N-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

19491-1-AP;HeLa cells

IP Result of anti-ATG14/Barkor (N-terminal) (IP:19491-1-AP, 4ug; Detection:19491-1-AP 1:500) with HeLa cells lysate 2800ug.

19491-1-AP;MCF-7 cells

Immunofluorescent analysis of MCF-7 cells using 19491-1-AP(ATG14/Barkor (N-terminal) antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, bovine, dog
Positive WB detected in:
mouse brain tissue, HEK-293 cells
Positive IP detected in:
HeLa cells
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:10-1:100

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
KIAA0831
Calculated molecular weight:
55 kDa
Observed molecular weight:
55-60 kDa
GenBank accession number:
NM_014924
Gene ID (NCBI):
22863
Gene symbol
KIAA0831
Synonyms
ATG14, Atg14L, Barkor, KIAA0831
Background

Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).


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