ATG14/Barkor (N-terminal) Antibody 5 Publications

Rabbit Polyclonal| Catalog number: 19491-1-AP

Featured Product KD/KO validated

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Reviews(1)


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Freight/Packing

Con: 52 μg/150 μl

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Species specificity:
human, mouse, rat, bovine, dog

Positive WB detected in:
mouse brain tissue, HEK-293 cells

Positive IP detected in:
HeLa cells

Positive IF detected in:
MCF-7 cells

Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Immunogen:
Peptide

Full name:
KIAA0831

Calculated molecular weight:
55 kDa

Observed molecular weight:
55-60 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
KIAA0831

Synonyms
ATG14, Atg14L, Barkor, KIAA0831
Background

Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).

ATG14/Barkor (N-terminal) Rabbit Polyclonal antibody

Charlotte Montespan, 01-25-2018

Applications
Immunofluorescence,
Lot Number
00011127

Primary Antibody Dilution
1:100
Secondary Antibody Dilution
1:500

Cell/Tissue Type
U2OS


I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.

Proteintech Response
Thank you for your review and bringing this to our attention. We will test this result in U2OS cells and refine this product after further investigation.

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