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ATG14/Barkor (N-terminal) Polyclonal antibody
ATG14/Barkor (N-terminal) Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse and More (3)
WB, IHC, IF, ELISA
Cat no : 19491-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells|
|Positive IHC detected in||mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 18 publications below|
|IF||See 2 publications below|
19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat, bovine, canine|
|Host / Isotype||Rabbit / IgG|
|Calculated molecular weight||55 kDa|
|Observed molecular weight||65-68 kDa|
|GenBank accession number||NM_014924|
|Gene ID (NCBI)||22863|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).
|Product Specific Protocols|
|WB protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP||Download protocol|
|IHC protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP||Download protocol|
|IF protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Signal Transduct Target Ther
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Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Charlotte (Verified Customer) (01-25-2018)
I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.