ATG14/Barkor (N-terminal) Polyclonal antibody

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  WB analysis of HEK-293 using 19491-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 19491-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 19491-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 19491-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19491-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19491-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 19491-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using 19491-1-AP (ATG14/Barkor (N-terminal) antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ATG14/Barkor (N-terminal) Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 19491-1-AP

Publications(34)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Barkor, ATG14, Beclin 1-associated autophagy-related key regulator, Autophagy-related protein 14-like protein, Atg14L

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 33 publications below
• IF: See 6 publications below
• IP: See 1 publications below

Product Information

19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, canine, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: KIAA0831
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: NM_014924
• Gene Symbol: ATG14
• Gene ID (NCBI): 22863
• RRID: AB_10642701
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZNE5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng
human	WB	Mol Cancer; PMID: 32727463; Authors - Cefan Zhou
human	WB	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
human	WB	Autophagy; STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.; Authors - Cefan Zhou
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	WB	Autophagy; Simultaneous inhibition of the ubiquitin-proteasome system and autophagy enhances apoptosis induced by ER stress aggravators in human pancreatic cancer cells.; Authors - Xu Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Charlotte (Verified Customer) (01-25-2018)

I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: U2OS

ATG14/Barkor (N-terminal) Antibody Immunofluorescence, validation (1:100 dilution) in U2OS (Cat no:19491-1-AP)