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ATG14/Barkor (N-terminal) Polyclonal antibody
ATG14/Barkor (N-terminal) Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (3)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 19491-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, mouse brain tissue, NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 12 publications below |
| IF | See 2 publications below |
Product Information
19491-1-AP targets ATG14/Barkor (N-terminal) in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | bovine, canine, human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | KIAA0831 |
| Calculated molecular weight | 55 kDa |
| Observed molecular weight | 65-68 kDa |
| GenBank accession number | NM_014924 |
| Gene symbol | KIAA0831 |
| Gene ID (NCBI) | 22863 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP | Download protocol |
| IHC protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP | Download protocol |
| IF protocol for ATG14/Barkor (N-terminal) antibody 19491-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Spatiotemporal Control of ULK1 Activation by NDP52 and TBK1 during Selective Autophagy. | ||
Mol Psychiatry Sequential formation of different layers of dystrophic neurites in Alzheimer's brains. | ||
J Microbiol Bovine viral diarrhea virus infection induces autophagy in MDBK cells.
| ||
Onco Targets Ther MicroRNA-375 Targets ATG14 to Inhibit Autophagy and Sensitize Hepatocellular Carcinoma Cells to Sorafenib. | ||
J Gen Virol Lentivirus-mediated bta-miR-29b overexpression interferes with bovine viral diarrhea virus replication and viral infection-related autophagy by directly targeting ATG14 and ATG9A in MDBK cells. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Charlotte (Verified Customer) (01-25-2018) | I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.
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