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ATG14/Barkor (N-terminal) Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 19491-1-AP

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Reviews(1)

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Con: 52 μg/150 μl

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Tested Applications

Applications:
WB, IP, IF, ELISA
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Images See all

ATG14/Barkor (N-terminal) Antibody WB mouse brain tissue 19491-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 19491-1-AP(ATG14/Barkor (N-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ATG14/Barkor (N-terminal) Antibody IP HeLa cells 19491-1-AP

IP Result of anti-ATG14/Barkor (N-terminal) (IP:19491-1-AP, 4ug; Detection:19491-1-AP 1:500) with HeLa cells lysate 2800ug.

ATG14/Barkor (N-terminal) Antibody IF MCF-7 cells 19491-1-AP

Immunofluorescent analysis of MCF-7 cells using 19491-1-AP(ATG14/Barkor (N-terminal) antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, bovine, dog
Positive WB detected in:
mouse brain tissue, HEK-293 cells
Positive IP detected in:
HeLa cells
Positive IF detected in:
MCF-7 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Peptide
Full name:
KIAA0831
Calculated molecular weight:
55 kDa
Observed molecular weight:
55-60 kDa
GenBank accession number:
NM_014924
Gene ID (NCBI):
22863
Gene symbol
KIAA0831
Synonyms
ATG14, Atg14L, Barkor, KIAA0831

Background

Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).

Customer Reviews

Share your scientific insight on this antibody

ATG14/Barkor (N-terminal) Rabbit Polyclonal antibody

Charlotte Montespan, 01-25-2018
Applications
Immunofluorescence,
Lot Number
00011127
Primary Antibody Dilution
1:100
Secondary Antibody Dilution
1:500
Cell/Tissue Type
U2OS

I do not think that this antibody works properly in U2OS cells. According to the litterature ATG14 is supposed to have a diffuse or punctae pattern and not this huge accumulation near the nucleus.
Proteintech Response
Thank you for your review and bringing this to our attention. We will test this result in U2OS cells and refine this product after further investigation.

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