Bcl2 Polyclonal antibody

• Featured Product
• KD/KO Validated

Bcl2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human and More (4)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

510

Reviews (2)

Cat no : 26593-1-AP

Synonyms

AW986256, B cell leukemia/lymphoma 2, Bcl2, Bcl-2, C430015F12Rik, D630044D05Rik, D830018M01Rik

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Validation Data Gallery

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  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 26593-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 26593-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of C2C12 using 26593-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 26593-1-AP (Bcl2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26593-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26593-1-AP (Bcl2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 26593-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Bcl2 antibody (26593-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of NIH/3T3 using 26593-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using 26593-1-AP (Bcl2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HL-60 cells, mouse brain tissue, C2C12 cells, Jurkat cells, mouse heart tissue, mouse testis tissue, mouse kidney tissue, mouse spleen tissue, rat spleen tissue, NIH3T3 cells, THP-1 cells, NIH/3T3 cells, U-937 cell, mouse kidney tissues
• Positive IHC detected in: human tonsillitis tissue, mouse spleen tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 491 publications below
• IHC: See 30 publications below
• IF: See 8 publications below

Product Information

26593-1-AP targets Bcl2 in WB, IHC, IF, ELISA applications and shows reactivity with mouse, rat, human samples.

• Tested Reactivity: mouse, rat, human
• Cited Reactivity: mouse, rat, canine, chicken, hamster, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Bcl2 fusion protein Ag24260
• Full Name: B-cell leukemia/lymphoma 2
• Calculated molecular weight: 26 kDa
• Observed molecular weight: 26 kDa
• GenBank accession number: NM-009741
• Gene symbol: Bcl2
• Gene ID (NCBI): 12043
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

1.       What is Bcl-2?

Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.

2.       FAQs for Bcl-2

1.  How to measure Bcl-2 and Bax apoptotic markers by western blotting

   Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
   

2. What band size should I expect in western blotting for Bcl-2?

   Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and       Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.

Publications

Species	Application	Title
mouse	WB	Adv Healthc Mater; GDNF-Loaded Polydopamine Nanoparticles-Based Anisotropic Scaffolds Promote Spinal Cord Repair by Modulating Inhibitory Microenvironment; Authors - Jinjin Ma
mouse	WB	Redox Biol; FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response; Authors - Zhe Wang
human,mouse	WB	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang
chicken	WB	J Hazard Mater; The role of ATF6 in Cr(VI)-induced apoptosis in DF-1 cells.; Authors - Shuo Zhang
mouse	WB	Theranostics; Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I.; Authors - Lujing Jiang
mouse,rat	WB	Free Radic Biol Med; The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro; Authors - Jie Ai

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Anna (Verified Customer) (10-04-2022)

Promising staining. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view

• Applications: Immunofluorescence
• Primary Antibody Dilution: 500
• Cell Tissue Type: brain frozen sections - mouse

FH

PRADEEP (Verified Customer) (11-03-2020)

Giving very good result for IF.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Mouse muscle