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Bcl2 Polyclonal antibody
Bcl2 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse, rat, human and More (4)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
510
Cat no : 26593-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HL-60 cells, mouse brain tissue, C2C12 cells, Jurkat cells, mouse heart tissue, mouse testis tissue, mouse kidney tissue, mouse spleen tissue, rat spleen tissue, NIH3T3 cells, THP-1 cells, NIH/3T3 cells, U-937 cell, mouse kidney tissues |
Positive IHC detected in | human tonsillitis tissue, mouse spleen tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | NIH/3T3 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 491 publications below |
IHC | See 30 publications below |
IF | See 8 publications below |
Product Information
26593-1-AP targets Bcl2 in WB, IHC, IF, ELISA applications and shows reactivity with mouse, rat, human samples.
Tested Reactivity | mouse, rat, human |
Cited Reactivity | mouse, rat, canine, chicken, hamster, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Bcl2 fusion protein Ag24260 |
Full Name | B-cell leukemia/lymphoma 2 |
Calculated molecular weight | 26 kDa |
Observed molecular weight | 26 kDa |
GenBank accession number | NM-009741 |
Gene symbol | Bcl2 |
Gene ID (NCBI) | 12043 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
1. What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2. FAQs for Bcl-2
How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
What band size should I expect in western blotting for Bcl-2?
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Bcl2 antibody 26593-1-AP | Download protocol |
IHC protocol for Bcl2 antibody 26593-1-AP | Download protocol |
IF protocol for Bcl2 antibody 26593-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Adv Healthc Mater GDNF-Loaded Polydopamine Nanoparticles-Based Anisotropic Scaffolds Promote Spinal Cord Repair by Modulating Inhibitory Microenvironment | ||
Redox Biol FUT2-dependent fucosylation of HYOU1 protects intestinal stem cells against inflammatory injury by regulating unfolded protein response | ||
Br J Pharmacol Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A | ||
Theranostics Proteomic analysis reveals ginsenoside Rb1 attenuates myocardial ischemia/reperfusion injury through inhibiting ROS production from mitochondrial complex I. | ||
Free Radic Biol Med The neuroprotective effects of phosphocreatine on amyloid beta 25-35-induced differentiated neuronal cell death through inhibition of AKT/GSK-3β /Tau/APP/CDK5 pathways in vivo and vitro |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Anna (Verified Customer) (10-04-2022) | Promising staining. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view
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FH PRADEEP (Verified Customer) (11-03-2020) | Giving very good result for IF.
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