Validation Data Gallery
|Positive WB detected in||NIH/3T3 cells, HL-60 cells, mouse brain tissue, C2C12 cells, Jurkat cells, mouse heart tissue, mouse testis tissue, U-937 cell, mouse kidney tissues, mouse spleen tissue, rat spleen tissue, rat kidney tissue, NIH3T3 cells, THP-1 cells, rat testis tissue|
|Positive IHC detected in||human tonsillitis tissue, mouse spleen tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Positive FC detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:500-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:800|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
26593-1-AP targets Bcl2 in WB, IHC, IF, FC, ELISA applications and shows reactivity with mouse, rat, human samples.
|Tested Reactivity||mouse, rat, human|
|Cited Reactivity||mouse, rat, chicken, hamster, pig, duck, canine, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Bcl2 fusion protein Ag24260|
|Full Name||B-cell leukemia/lymphoma 2|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||26 kDa|
|GenBank accession number||NM-009741|
|Gene ID (NCBI)||12043|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
1. What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2. FAQs for Bcl-2
How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Anna (Verified Customer) (10-04-2022)
Promising staining. I have a problem with attaching a photo file. Please check the file below. https://drive.google.com/file/d/15d3t6thx7rcWyN-_DkkKCtpAMyi8jxHO/view
PRADEEP (Verified Customer) (11-03-2020)
Giving very good result for IF.