Beclin 1 Monoclonal antibody

Beclin 1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (4)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

12

CloneNo.

1C10C4

Cat no : 66665-1-Ig

Synonyms

ATG6, Beclin 1, beclin 1, autophagy related, beclin1, BECN1, GT197, Protein GT197, VPS30

---

Validation Data Gallery

• 

  WB analysis using 66665-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66665-1-Ig (Beclin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 66665-1-Ig

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:500 (under 10x lens).

• 

  IF Staining of mouse heart using 66665-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse heart using 66665-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 66665-1-Ig (Beclin 1 antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, RAW 264.7 cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, LNCaP cells, MCF-7 cells, HEK-293 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 8 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Product Information

66665-1-Ig targets Beclin 1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: goat, human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Beclin 1 fusion protein Ag1843
• Full Name: beclin 1, autophagy related
• Calculated molecular weight: 52 kDa
• Observed molecular weight: 60 kDa
• GenBank accession number: BC010276
• Gene symbol: BECN1
• Gene ID (NCBI): 8678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.

Publications

Species	Application	Title
human	IHC	Hum Exp Toxicol; Beclin 1, LC3, and p62 expression in paraquat-induced pulmonary fibrosis.; Authors - G Xu
human	WB	Oxid Med Cell Longev; DpdtC-Induced EMT Inhibition in MGC-803 Cells Was Partly through Ferritinophagy-Mediated ROS/p53 Pathway.; Authors - Jiankang Feng
mouse	WB	Int J Mol Sci; Moderate, but Not Excessive, Training Attenuates Autophagy Machinery in Metabolic Tissues.; Authors - Alisson L da Rocha
goat	WB	Oxid Med Cell Longev; Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.; Authors - Tian-Yu Feng
human	WB,IHC	Drug Des Devel Ther; Homoharringtonine Exerts an Antimyeloma Effect by Promoting Excess Parkin-Dependent Mitophagy.; Authors - Yanyu Zhang
human	WB	Chem Biol Interact; The vicious cycle between ferritinophagy and ROS production triggered EMT inhibition of gastric cancer cells was through p53/AKT/mTor pathway.; Authors - Zhongjie Xu