Beclin 1 Monoclonal antibody

Beclin 1 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat and More (4)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1C10C4

Cat no : 66665-1-Ig

Synonyms

ATG6, Beclin 1, beclin 1, autophagy related, beclin1, BECN1, GT197, Protein GT197, VPS30



Tested Applications

Positive WB detected inHeLa cells, RAW 264.7 cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells, LNCaP cells, MCF-7 cells, HEK-293 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66665-1-Ig targets Beclin 1 in WB, IHC, IF,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Cited Reactivity goat, human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Beclin 1 fusion protein Ag1843
Full Name beclin 1, autophagy related
Calculated molecular weight 52 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC010276
Gene symbol BECN1
Gene ID (NCBI) 8678
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Beclin 1, also known as ATG6 or VPS30, interacts with various cofactors (e.g. Ambra1, Barkor (Atg14), Rubicon, or UVRAG) to regulate the lipid kinase Vps34 and promote the formation of the BECLIN1-Vps34-Vps15 complex, hence inducing autophagy. Its function (via the BH3 domain) is inhibited by Bcl-2 or Bcl-XL. Beclin 1 (BECN1) is a crucial molecule in the control of the autophagic activity, and its activity is regulated by multiple mechanisms, including the post-translational modification, protein-protein interaction, and subcellular localization. It plays a role in crosstalk between apoptosis and autophagy. It has been reported that Beclin 1 can be cleaved into fragments of 50, 37 and 35 kDa during apoptosis. It is involved in many disorders, including neurodegeneration and cancer (tumorigenesis). Beclin 1 is a mammalian tumor suppressor, and its gene is monoallelically deleted in 75% of ovarian, 50% of breast, and 40% of prostate cancers. Decreased expression of Beclin 1 has also been observed in human brain and lung tumors. The level of Beclin 1 was decreased in the affected brain regions of patients with Alzheimer's disease early in the disease process. Recent studies have also shown that gain and loss of Beclin 1 function affects the death of heart cells.

Protocols

Product Specific Protocols
WB protocol for Beclin 1 antibody 66665-1-IgDownload protocol
IHC protocol for Beclin 1 antibody 66665-1-IgDownload protocol
IF protocol for Beclin 1 antibody 66665-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Hum Exp Toxicol

Beclin 1, LC3, and p62 expression in paraquat-induced pulmonary fibrosis.

Authors - G Xu
humanWB

Oxid Med Cell Longev

DpdtC-Induced EMT Inhibition in MGC-803 Cells Was Partly through Ferritinophagy-Mediated ROS/p53 Pathway.

Authors - Jiankang Feng
mouseWB

Int J Mol Sci

Moderate, but Not Excessive, Training Attenuates Autophagy Machinery in Metabolic Tissues.

Authors - Alisson L da Rocha
goatWB

Oxid Med Cell Longev

Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.

Authors - Tian-Yu Feng
humanWB,IHC

Drug Des Devel Ther

Homoharringtonine Exerts an Antimyeloma Effect by Promoting Excess Parkin-Dependent Mitophagy.

Authors - Yanyu Zhang
humanWB

Chem Biol Interact

The vicious cycle between ferritinophagy and ROS production triggered EMT inhibition of gastric cancer cells was through p53/AKT/mTor pathway.

Authors - Zhongjie Xu