Prestained Protein Marker

Molecular Weight

3-245 kDa

Detection Method

Colorimetric

Number of Markers

13

Stain Type

3 colors: Blue, Red, Green

Reviews (1)

Cat no : PL00002



Product Information

The PL00002 Broad Range Protein Marker is a ready-to-use three-color protein standard with 13 prestained proteins covering a wide range of molecular weights from 3 to 245 kDa in Tris-Glycine buffer. The PL00002 Broad Range Prestained Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for estimating the size of proteins.

Detection Method Colorimetric
Molecular Weight ~ 3, 10, 15, 20, 25, 35, 45, 60, 75, 100, 140, 180, 245 kDa
Number of Markers 13
Size Range 3 to 245
Stain Type 3 colors: Blue, Red, Green
Contents Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Storage Store product at 4 C for up to 12 weeks. For longer storage, aliquot and store at -20 C for up to 1 year.

*This product may ship at ambient temperature. Upon receiving this product, immediately store product at the recommended temperatures.

Instructions The protein ladder comes ready to use in gel loading buffer. After thawing and mixing, load approximately 3-5 μL of protein marker per lane. Do NOT heat, dilute, or add reducing agents before loading.

Reviews

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