C1QBP Polyclonal antibody

• Featured Product
• KD/KO Validated

C1QBP Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

7

Reviews (1)

Cat no : 24474-1-AP

Synonyms

C1QBP, gC1Q R, GC1q R protein, GC1QBP, gC1qR, Glycoprotein gC1qBP, HABP1, Hyaluronan binding protein 1, p32, p33, SF2p32

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Validation Data Gallery

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  WB analysis using 24474-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24474-1-AP (C1QBP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24474-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24474-1-AP (C1QBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 24474-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24474-1-AP (C1QBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 24474-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24474-1-AP (C1QBP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 24474-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24474-1-AP (C1QBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 24474-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24474-1-AP (C1QBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24474-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24474-1-AP (C1QBP Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 24474-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 24474-1-AP (C1QBP Antibody) at dilution of 1:50.

• 

  IF Staining of NIH/3T3 using 24474-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 24474-1-AP (C1QBP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 24474-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human C1QBP (24474-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: C2C12 cells, K-562 cells, NIH/3T3 cells, HeLa cells, HCT 116 cells, PC-12 cells, RAW 264.7 cells
• Positive IHC detected in: human tonsillitis tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IF: See 3 publications below

Product Information

The immunogen of 24474-1-AP is C1QBP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C1QBP fusion protein Ag19773
• Full Name: complement component 1, q subcomponent binding protein
• Calculated molecular weight: 282 aa, 31 kDa
• Observed molecular weight: 32 kDa
• GenBank accession number: BC013731
• Gene symbol: C1QBP
• Gene ID (NCBI): 708
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).

Publications

Species	Application	Title
human	WB,IF	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang ; KO Validated
human	WB	Proc Natl Acad Sci U S A; A nucleolar long "non-coding" RNA encodes a novel protein that functions in response to stress; Authors - Shuang Feng
human	WB	J Control Release; PSMA-targeted nanoparticles for specific penetration of blood-brain tumor barrier and combined therapy of brain metastases.; Authors - Jiang Ni
mouse	WB	Cancer Sci; Complement C1q binding protein regulates T cells' mitochondrial fitness to affect their survival, proliferation, and anti-tumor immune function.; Authors - Hui Tian ; KO Validated
	WB,IF	Int J Mol Sci; The Expression Pattern of p32 in Sheep Muscle and Its Role in Differentiation, Cell Proliferation, and Apoptosis of Myoblasts.; Authors - Jianyu Ma ; KD Validated
rat	WB	Biol Pharm Bull; Proteomic analysis reveals the renoprotective effect of Tribulus terrestris against obesity-related glomerulopathy in rats.; Authors - Yue-Hua Jiang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Lana (Verified Customer) (08-13-2019)

SDS-PAGE: 15 ug/ul RIPA lysate of the mitochondrial fraction of HEK293t. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1h.Primary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293t

C1QBP Antibody Western Blot, validation (1:500 dilution) in HEK293t (Cat no:24474-1-AP)

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