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  • KD/KO Validated

C1QBP Polyclonal antibody

C1QBP Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 24474-1-AP

Synonyms

C1QBP, gC1Q R, GC1q R protein, GC1QBP, gC1qR, Glycoprotein gC1qBP, HABP1, Hyaluronan binding protein 1, p32, p33, SF2p32



Tested Applications

Positive WB detected inC2C12 cells, K-562 cells, NIH/3T3 cells, HeLa cells, HCT 116 cells, PC-12 cells, RAW 264.7 cells
Positive IHC detected inhuman tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inNIH/3T3 cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24474-1-AP is C1QBP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C1QBP fusion protein Ag19773
Full Name complement component 1, q subcomponent binding protein
Calculated molecular weight 282 aa, 31 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC013731
Gene symbol C1QBP
Gene ID (NCBI) 708
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).

Protocols

Product Specific Protocols
WB protocol for C1QBP antibody 24474-1-APDownload protocol
IHC protocol for C1QBP antibody 24474-1-APDownload protocol
IF protocol for C1QBP antibody 24474-1-APDownload protocol
FC protocol for C1QBP antibody 24474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Biol Pharm Bull

Proteomic analysis reveals the renoprotective effect of Tribulus terrestris against obesity-related glomerulopathy in rats.

Authors - Yue-Hua Jiang
WB,IF

Int J Mol Sci

The Expression Pattern of p32 in Sheep Muscle and Its Role in Differentiation, Cell Proliferation, and Apoptosis of Myoblasts.

Authors - Jianyu Ma
  • KD Validated
humanWB

J Control Release

PSMA-targeted nanoparticles for specific penetration of blood-brain tumor barrier and combined therapy of brain metastases.

Authors - Jiang Ni

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lana (Verified Customer) (08-13-2019)

SDS-PAGE: 15 ug/ul RIPA lysate of the mitochondrial fraction of HEK293t. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1h.Primary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HEK293t
C1QBP Antibody Western Blot, validation (1:500 dilution) in HEK293t (Cat no:24474-1-AP)