Validation Data Gallery
|Positive WB detected in||C2C12 cells, K-562 cells, NIH/3T3 cells, HeLa cells, HCT 116 cells, PC-12 cells, RAW 264.7 cells|
|Positive IHC detected in||human tonsillitis tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24474-1-AP is C1QBP Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C1QBP fusion protein Ag19773|
|Full Name||complement component 1, q subcomponent binding protein|
|Calculated molecular weight||282 aa, 31 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC013731|
|Gene ID (NCBI)||708|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
C1QBP, also named as gC1q receptor (gC1qR), p32, p33, and hyaluronan-binding protein 1 (HABP1), is a protein initially copurified with splicing factor SF2 (PMID: 1830244). The protein is synthesized as a pro-protein of 282 amino acids (aa) that is post-translationally processed by removal of the initial 73 aa to a mature protein of 209 aa (PMID: 8262387). C1QBP is an evolutionary conserved and ubiquitously expressed multifunctional protein and has been reported to be a predominantly mitochondrial matrix protein involved in inflammation and infection processes, mitochondrial ribosome biogenesis, regulation of apoptosis and nuclear transcription, and pre-mRNA splicing (PMID: 28942965).
|Product Specific Protocols|
|WB protocol for C1QBP antibody 24474-1-AP||Download protocol|
|IHC protocol for C1QBP antibody 24474-1-AP||Download protocol|
|IF protocol for C1QBP antibody 24474-1-AP||Download protocol|
|FC protocol for C1QBP antibody 24474-1-AP||Download protocol|
|Click here to view our Standard Protocols|
C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse
Proc Natl Acad Sci U S A
A nucleolar long "non-coding" RNA encodes a novel protein that functions in response to stress
J Control Release
PSMA-targeted nanoparticles for specific penetration of blood-brain tumor barrier and combined therapy of brain metastases.
Complement C1q binding protein regulates T cells' mitochondrial fitness to affect their survival, proliferation, and anti-tumor immune function.
Int J Mol Sci
The Expression Pattern of p32 in Sheep Muscle and Its Role in Differentiation, Cell Proliferation, and Apoptosis of Myoblasts.
Biol Pharm Bull
Proteomic analysis reveals the renoprotective effect of Tribulus terrestris against obesity-related glomerulopathy in rats.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lana (Verified Customer) (08-13-2019)
SDS-PAGE: 15 ug/ul RIPA lysate of the mitochondrial fraction of HEK293t. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4C.Blocking: SEA Block Blocking Buffer 1h.Primary Ab: O/N incubation at 4C.Secondary Ab: IRDye 800CW Goat anti-Rabbit.