C2orf69 Polyclonal antibody

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  WB analysis using 32100-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32100-1-AP (C2orf69 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 32100-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 32100-1-AP (C2orf69 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

C2orf69 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 32100-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Chromosome 2 Open Reading Frame 69, COXPD53, FLJ38973, Hypothetical Protein FLJ38973, Mitochondrial protein C2orf69

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Tested Applications

• Positive WB detected in: mouse stomach tissue, rat stomach tissue
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32100-1-AP targets C2orf69 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: C2orf69 fusion protein Ag36779
• Full Name: chromosome 2 open reading frame 69
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC036456
• Gene Symbol: C2orf69
• Gene ID (NCBI): 205327
• RRID: AB_3670190
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q8N8R5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Chromosome 2 open reading frame 69 (C2orf69, also known as COXPD53) is located in the mitochondrion. It can affect mitochondrial membrane potential and oxidative respiration in cultured neurons and controls the levels of the glycogen branching enzyme 1 (GBE1) (PMID: 34038740; 33945503). The variant in C2orf69 probably causes developmental regression, seizures, microcephaly, autistic features, and hypertonia (PMID: 37337918). Its deficiency can disrupt the development and homeostasis of the immune and central nervous systems (PMID: 34038740).