MUC1/CA15-3 C-terminal Polyclonal antibody
MUC1/CA15-3 C-terminal Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, FC
Conjugate
Unconjugated
Cat no : 23614-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | DU 145 cells, PC-3 cells, BxPC-3 cells, MCF-7 cells |
Positive IHC detected in | human breast cancer tissue, human ovary tumor tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | MCF-7 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
FC | See 1 publications below |
Product Information
The immunogen of 23614-1-AP is MUC1/CA15-3 C-terminal Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MUC1/CA15-3 C-terminal fusion protein Ag20366 |
Full Name | mucin 1, cell surface associated |
Calculated molecular weight | 1264 aa, 123 kDa |
Observed molecular weight | 23-28 kDa |
GenBank accession number | BC120975 |
Gene symbol | MUC1 |
Gene ID (NCBI) | 4582 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.
Protocols
Product Specific Protocols | |
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WB protocol for MUC1/CA15-3 C-terminal antibody 23614-1-AP | Download protocol |
IHC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Death Discov Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion. | ||
ACS Sens Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins. |