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- KD/KO Validated
MUC1/CA15-3 C-terminal Polyclonal antibody
MUC1/CA15-3 C-terminal Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human and More (1)
WB, IHC, FC, ELISA
Cat no : 23614-1-AP
|Positive WB detected in||DU 145 cells, PC-3 cells, BxPC-3 cells, MCF-7 cells|
|Positive IHC detected in||human breast cancer tissue, human ovary tumor tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 6 publications below|
|IHC||See 2 publications below|
|FC||See 1 publications below|
23614-1-AP targets MUC1/CA15-3 C-terminal in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MUC1/CA15-3 C-terminal fusion protein Ag20366|
|Full Name||mucin 1, cell surface associated|
|Calculated molecular weight||1264 aa, 123 kDa|
|Observed molecular weight||23-28 kDa|
|GenBank accession number||BC120975|
|Gene ID (NCBI)||4582|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.
|Product Specific Protocols|
|WB protocol for MUC1/CA15-3 C-terminal antibody 23614-1-AP||Download protocol|
|IHC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Pathol Res Pract
MUC1 promotes cancer stemness and predicts poor prognosis in osteosarcoma
Cell Death Discov
Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.
Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.
Cell Death Discov
Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling.
LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway.
A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma