MUC1/CA15-3 C-terminal Polyclonal antibody

MUC1/CA15-3 C-terminal Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 23614-1-AP


CA15 3, CA153, CA15-3, Carcinoma associated mucin, CD227, EMA, Episialin, H23AG, KL-6, MAM6, MUC 1, MUC1, MUC1 beta, MUC1 CT, MUC1 NT, MUC1/CA15-3 C-terminal, Mucin 1, Peanut reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor associated mucin

Tested Applications

Positive WB detected inDU 145 cells, PC-3 cells, BxPC-3 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23614-1-AP is MUC1/CA15-3 C-terminal Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC1/CA15-3 C-terminal fusion protein Ag20366
Full Name mucin 1, cell surface associated
Calculated molecular weight 1264 aa, 123 kDa
Observed molecular weight 23-28 kDa
GenBank accession numberBC120975
Gene symbol MUC1
Gene ID (NCBI) 4582
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.


Product Specific Protocols
WB protocol for MUC1/CA15-3 C-terminal antibody 23614-1-APDownload protocol
IHC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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