MUC1/CA15-3 C-terminal Polyclonal antibody

MUC1/CA15-3 C-terminal Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 23614-1-AP

Synonyms

CA15 3, CA153, CA15-3, Carcinoma associated mucin, CD227, EMA, Episialin, H23AG, KL-6, MAM6, MUC 1, MUC1, MUC1 beta, MUC1 CT, MUC1 NT, MUC1/CA15-3 C-terminal, Mucin 1, Peanut reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor associated mucin



Tested Applications

Positive WB detected inMCF-7 cells, BxPC-3 cells
Positive IHC detected inhuman ovary tumor tissue, human tonsillitis tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23614-1-AP is MUC1/CA15-3 C-terminal Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MUC1/CA15-3 C-terminal fusion protein Ag20366
Full Name mucin 1, cell surface associated
Calculated molecular weight 1264 aa, 123 kDa
Observed molecular weight 23-28 kDa
GenBank accession numberBC120975
Gene symbol MUC1
Gene ID (NCBI) 4582
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.

Protocols

Product Specific Protocols
WB protocol for MUC1/CA15-3 C-terminal antibody 23614-1-APDownload protocol
IHC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-APDownload protocol
FC protocol for MUC1/CA15-3 C-terminal antibody 23614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Death Discov

Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.

Authors - Wenwen Wang