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- KD/KO Validated
MUC1/CA15-3 C-terminal Polyclonal antibody
MUC1/CA15-3 C-terminal Polyclonal Antibody for FC, IHC, WB, ELISA
Cat no : 23614-1-AP
Validation Data Gallery
|Positive WB detected in||DU 145 cells, PC-3 cells, BxPC-3 cells, MCF-7 cells|
|Positive IHC detected in||human pancreas cancer tissue, human tonsillitis tissue, human breast cancer tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||MCF-7 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
23614-1-AP targets MUC1/CA15-3 C-terminal in WB, IHC, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MUC1/CA15-3 C-terminal fusion protein Ag20366|
|Full Name||mucin 1, cell surface associated|
|Calculated molecular weight||1264 aa, 123 kDa|
|Observed molecular weight||23-28 kDa|
|GenBank accession number||BC120975|
|Gene ID (NCBI)||4582|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.
Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.
LINC02535/miR-30a-5p/GALNT3 axis contributes to lung adenocarcinoma progression via the NF- κ B signaling pathway.
Pathol Res Pract
MUC1 promotes cancer stemness and predicts poor prognosis in osteosarcoma
Cell Death Discov
Silencing of lncRNA MALAT1 facilitates erastin-induced ferroptosis in endometriosis through miR-145-5p/MUC1 signaling.
Cell Death Discov
Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.
A ferroptosis-related gene signature associated with immune landscape and therapeutic response in osteosarcoma