MUC1/CA15-3 C-terminal Polyclonal antibody

MUC1/CA15-3 C-terminal Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, FC

Conjugate

Unconjugated

Publications

2

Cat no : 23614-1-AP

Synonyms

CA15 3, CA153, CA15-3, Carcinoma associated mucin, CD227, EMA, Episialin, H23AG, KL-6, MAM6, MUC 1, MUC1, MUC1 beta, MUC1 CT, MUC1 NT, MUC1/CA15-3 C-terminal, Mucin 1, Peanut reactive urinary mucin, PEM, PEMT, Polymorphic epithelial mucin, PUM, Tumor associated mucin

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Validation Data Gallery

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  WB analysis using 23614-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23614-1-AP (MUC1/CA15-3 C-terminal antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 23614-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23614-1-AP (MUC1/CA15-3 C-terminal antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human tonsillitis using 23614-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23614-1-AP (MUC1/CA15-3 C-terminal antibody) at dilution of 1:1600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 23614-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 23614-1-AP (MUC1/CA15-3 antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of MCF-7 using 23614-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug MUC1/CA15-3 antibody (23614-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: DU 145 cells, PC-3 cells, BxPC-3 cells, MCF-7 cells
• Positive IHC detected in: human breast cancer tissue, human ovary tumor tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• FC: See 1 publications below

Product Information

The immunogen of 23614-1-AP is MUC1/CA15-3 C-terminal Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MUC1/CA15-3 C-terminal fusion protein Ag20366
• Full Name: mucin 1, cell surface associated
• Calculated molecular weight: 1264 aa, 123 kDa
• Observed molecular weight: 23-28 kDa
• GenBank accession number: BC120975
• Gene symbol: MUC1
• Gene ID (NCBI): 4582
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MUC1 is a type I transmembrane glycoprotein expressed by various epithelial cells of female reproductive tract, lung, breast, kidney, stomach, and pancreas. MUC1 is transcribed as a large precursor gene product, and upon translation, is cleaved in the endoplasmic reticulum, yielding two subunits: the large extracellular N-terminal subunit (MUC1-N, about 120-200 kDa) and the small cytoplasmic C-terminal subunit (MUC1-C, about 23-30 kDa). Among the known mucins, MUC1 is best studied and plays crucial roles in regulating many cellular properties, including cell proliferation, apoptosis, adhesion, and invasion. MUC1 is overexpressed in a wide range of human epithelial malignancies. This antibody was raised against the C-terminal region of human MUC1, thus it recognizes the 23-30 kDa cytoplasmic MUC1.

Publications

Species	Application	Title
mouse	WB	Cell Death Discov; Loss of QKI in macrophage aggravates inflammatory bowel disease through amplified ROS signaling and microbiota disproportion.; Authors - Wenwen Wang
human	WB,FC	ACS Sens; Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins.; Authors - Xiaoyi Gao

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