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  • KD/KO Validated

CA3 Polyclonal antibody

CA3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15197-1-AP

Synonyms

CA III, CA3, CAIII, Car3, Carbonate dehydratase III, Carbonic anhydrase 3, Carbonic anhydrase III



Tested Applications

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, HeLa cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue, human kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15197-1-AP targets CA3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CA3 fusion protein Ag7344
Full Name carbonic anhydrase III, muscle specific
Calculated molecular weight 29 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC004897
Gene symbol CA3
Gene ID (NCBI) 761
RRIDAB_2243603
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).

Protocols

Product Specific Protocols
WB protocol for CA3 antibody 15197-1-APDownload protocol
IHC protocol for CA3 antibody 15197-1-APDownload protocol
IF protocol for CA3 antibody 15197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IHC

J Nutr Biochem

Postnatal high-fat diet sex-specifically exacerbates prenatal dexamethasone-induced hypertension: Mass spectrometry-based quantitative proteomic approach.

Authors - Chien-Ning Hsu
mouseIF

Am J Physiol Renal Physiol

Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA+ interstitial cells are fibroblasts

Authors - Dennis R. Clayton

Clin Proteomics

Orthogonal proteomics methods warrant the development of Duchenne muscular dystrophy biomarkers

Authors - Camilla Johansson
mouseWB

PLoS One

Multifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration.

Authors - Yuan Wang
  • KD Validated
mouseIHC

PLoS One

Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.

Authors - Claes-Göran Reibring
human,ratWB

FEBS Open Bio

Carbonic anhydrase 3 increases during liver adipogenesis even in pre-obesity, and its inhibitors reduce liver adipose accumulation.

Authors - Hiroyuki Yamamoto

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elise (Verified Customer) (06-21-2022)

Do not Work for IF with blocking serum 5% and antibody in BSA 1%. Will be tested on IHC and WB and Tested again in IF with 1: 150 dilution

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: kidney tissue