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CA3 Polyclonal antibody
CA3 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IHC, IF, ELISA
Cat no : 15197-1-AP
Validation Data Gallery
|Positive WB detected in||HEK-293 cells, mouse skeletal muscle tissue, rat skeletal muscle tissue, HeLa cells|
|Positive IHC detected in||mouse skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse skeletal muscle tissue, human kidney tissue|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|KD/KO||See 1 publications below|
|WB||See 3 publications below|
|IHC||See 2 publications below|
|IF||See 1 publications below|
15197-1-AP targets CA3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CA3 fusion protein Ag7344|
|Full Name||carbonic anhydrase III, muscle specific|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||30 kDa|
|GenBank accession number||BC004897|
|Gene ID (NCBI)||761|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Carbonic anhydrase III (CA3), which belongs to the alpha-carbonic anhydrase family, is a cytoplasmic enzyme that exhibits a relatively low carbon dioxide hydratase activity. It is expressed at a very high level in skeletal muscle, where physical exercise has been shown to increase free radical production. In addition to its carbon dioxide hydratase activity, CA3 has been demonstrated to have a carboxyl esterase activity and phosphatase activity, which suggests that it is a tyrosine phosphatase (PMID: 10064618). CA3 was found to be localized in Type-I muscle fibers and could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases (PMID: 6221502).
|Product Specific Protocols|
|WB protocol for CA3 antibody 15197-1-AP||Download protocol|
|IHC protocol for CA3 antibody 15197-1-AP||Download protocol|
|IF protocol for CA3 antibody 15197-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Nutr Biochem
Postnatal high-fat diet sex-specifically exacerbates prenatal dexamethasone-induced hypertension: Mass spectrometry-based quantitative proteomic approach.
Am J Physiol Renal Physiol
Studies of ultrastructure, gene expression, and marker analysis reveal that mouse bladder PDGFRA+ interstitial cells are fibroblasts
Multifactorial Comparative Proteomic Study of Cytochrome P450 2E1 Function in Chronic Alcohol Administration.
Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.
FEBS Open Bio
Carbonic anhydrase 3 increases during liver adipogenesis even in pre-obesity, and its inhibitors reduce liver adipose accumulation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Elise (Verified Customer) (06-21-2022)
Do not Work for IF with blocking serum 5% and antibody in BSA 1%. Will be tested on IHC and WB and Tested again in IF with 1: 150 dilution