CADM1 Polyclonal antibody, PBS Only

• 

  WB analysis of mouse liver using 14335-1-AP (same clone as 14335-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 14335-1-AP (same clone as 14335-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 14335-1-AP (same clone as 14335-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse lung using 14335-1-AP (same clone as 14335-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 14335-1-AP (CADM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 14335-1-AP (same clone as 14335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14335-1-AP (CADM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 14335-1-AP (same clone as 14335-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 14335-1-AP (CADM1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14335-1-PBS in a different storage buffer formulation.

CADM1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14335-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

NECL-2, NECL2, Necl 2, IGSF4A, IgSF4

---

Product Information

14335-1-PBS targets CADM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CADM1 fusion protein Ag5621
• Full Name: cell adhesion molecule 1
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49-60 kDa
• GenBank Accession Number: BC047021
• Gene Symbol: CADM1
• Gene ID (NCBI): 23705
• RRID: AB_2243801
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BY67
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CADM1 (cell adhesion molecule 1), also referred to as SgIGSF, Necl-2 or TSLC-1, is a cell adhesion molecule belonging to the immunoglobulin superfamily. It can mediate homophilic and heterophilic cell-cell adhesion. CADM1 is expressed by a variety of cells including neurons, mast cells, spermatogonia, pancreatic islet cells, epithelial cells such as lung alveolar cells and biliary epithelial cells. CADM1 is involved in carcinoma pathogenesis and the expression of CADM1 is frequently down-regulated in various tumors derived from epithelial cells. It acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. (PMID: 18332875; 17326163; 20340131)