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CAMKK2 Polyclonal antibody

CAMKK2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11549-1-AP

Synonyms

CaM kinase kinase 2, CaM kinase kinase beta, CaM KK 2, CaM KK beta, CAMKK, CaMKK 2, CaMKK beta, CAMKK2, CAMKKB, KIAA0787



Tested Applications

Positive WB detected inPC-3 cells, fetal human brain tissue, HeLa cells, human brain tissue, human heart tissue, Jurkat cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11549-1-AP targets CAMKK2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAMKK2 fusion protein Ag2093
Full Name calcium/calmodulin-dependent protein kinase kinase 2, beta
Calculated molecular weight 588 aa, 60 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC026060
Gene symbol CAMKK2
Gene ID (NCBI) 10645
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAMKK2(calcium/calmodulin-dependent protein kinase kinase 2) is also named as CAMKKB, KIAA0787 and belongs to the protein kinase superfamily. It regulates hypothalamic production of the orexigenic hormone NPY and contributes to the regulation of energy balance. CAMKK2 in vivo reduces tumor growth suggests that reactivation of CAMKK2 by development of new drugs may be a new strategy to prolong the period to respond androgen-deprivation therapy(ADT)(PMID:22549914). It can be autophosphorylated(PMID:19369195). It has 7 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for CAMKK2 antibody 11549-1-APDownload protocol
IHC protocol for CAMKK2 antibody 11549-1-APDownload protocol
IF protocol for CAMKK2 antibody 11549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Chin J Integr Med

Protective Effects of Combination of Radix Astragali and Radix Salviae Miltiorrhizae on Kidney of Spontaneously Hypertensive Rats and Renal Intrinsic Cells.

Authors - Wei Li
mouseWB

Pharm Biol

Berberine attenuates fructose-induced insulin resistance by stimulating the hepatic LKB1/AMPK/PGC1α pathway in mice.

Authors - Yucheng Li
mouseWB

Cell Physiol Biochem

Baicalin Attenuates High Fat Diet-Induced Obesity and Liver Dysfunction: Dose-Response and Potential Role of CaMKKβ/AMPK/ACC Pathway.

Authors - Youli Xi
ratWB

Toxicology

Carbon disulfide activates p62-Nrf2-keap1 pathway in rat nerve tissues.

Authors - Shasha Wang
ratWB

Nutrients

PMID: 30081580

Authors - Yu-Cheng Li
humanWB

Biochem J

Eukaryotic elongation factor 2 kinase regulates the cold stress response by slowing translation elongation.

Authors - John Rp Knight