CAMKK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CAMKK2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

24

Cat no : 11549-1-AP

Synonyms

CaM kinase kinase 2, CaM kinase kinase beta, CaM KK 2, CaM KK beta, CAMKK, CaMKK 2, CaMKK beta, CAMKK2, CAMKKB, KIAA0787

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Validation Data Gallery

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  WB analysis of PC-3 using 11549-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 11549-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11549-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 11549-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 11549-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11549-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 11549-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of PC-3 using 11549-1-AP

  Immunofluorescent analysis of PC-3 cells using 11549-1-AP (CAMKK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells, fetal human brain tissue, HeLa cells, human brain tissue, human heart tissue, Jurkat cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 5 publications below
• WB: See 21 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

11549-1-AP targets CAMKK2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAMKK2 fusion protein Ag2093
• Full Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
• Calculated molecular weight: 588 aa, 60 kDa
• Observed molecular weight: 55-60 kDa
• GenBank accession number: BC026060
• Gene symbol: CAMKK2
• Gene ID (NCBI): 10645
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CAMKK2(calcium/calmodulin-dependent protein kinase kinase 2) is also named as CAMKKB, KIAA0787 and belongs to the protein kinase superfamily. It regulates hypothalamic production of the orexigenic hormone NPY and contributes to the regulation of energy balance. CAMKK2 in vivo reduces tumor growth suggests that reactivation of CAMKK2 by development of new drugs may be a new strategy to prolong the period to respond androgen-deprivation therapy(ADT)(PMID:22549914). It can be autophosphorylated(PMID:19369195). It has 7 isoforms produced by alternative splicing.

Publications

Species	Application	Title
rat	WB	Chin J Integr Med; Protective Effects of Combination of Radix Astragali and Radix Salviae Miltiorrhizae on Kidney of Spontaneously Hypertensive Rats and Renal Intrinsic Cells.; Authors - Wei Li
mouse	WB	Biol Pharm Bull; Yi-Zhi-Fang-Dai Formula Exerts a Protective Effect on the Injury of Tight Junction Scaffold Proteins in Vitro and in Vivo by Mediating Autophagy through Regulation of the RAGE/CaMKKβ/AMPK/mTOR Pathway.; Authors - Yuanjin Chan
mouse	WB	Pharm Biol; Berberine attenuates fructose-induced insulin resistance by stimulating the hepatic LKB1/AMPK/PGC1α pathway in mice.; Authors - Yucheng Li
mouse	WB	Cell Physiol Biochem; Baicalin Attenuates High Fat Diet-Induced Obesity and Liver Dysfunction: Dose-Response and Potential Role of CaMKKβ/AMPK/ACC Pathway.; Authors - Youli Xi
rat	WB	Toxicology; Carbon disulfide activates p62-Nrf2-keap1 pathway in rat nerve tissues.; Authors - Shasha Wang
human	IF	Biochim Biophys Acta Mol Cell Res; Calcium/calmodulin-dependent protein kinase kinase 2 mediates pleiotropic effects of epidermal growth factor in cancer cells.; Authors - Shuhang Dai