CAMKK2 Polyclonal antibody

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  WB analysis of Jurkat using 11549-1-AP

  WB result of CAMKK2 antibody (11549-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CAMKK2 transfected Jurkat cells.

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  WB analysis using 11549-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11549-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 11549-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11549-1-AP (CAMKK2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 11549-1-AP

  IP result of anti-CAMKK2 (IP:11549-1-AP, 4ug; Detection:11549-1-AP 1:500) with PC-3 cells lysate 960 ug.

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  IHC staining of human prostate cancer using 11549-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human prostate cancer using 11549-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11549-1-AP (CAMKK2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of PC-3 using 11549-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CAMKK2 antibody (11549-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

CAMKK2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11549-1-AP

Publications(51)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

CaM KK beta, CaM KK 2, CaM kinase kinase beta, CaM kinase kinase 2, Calcium/calmodulin-dependent protein kinase kinase beta

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Tested Applications

• Positive WB detected in: Jurkat cells, fetal human brain tissue, HeLa cells, PC-3 cells, RAW 264.7 cells
• Positive IP detected in: PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 9 publications below
• WB: See 46 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• CoIP: See 1 publications below

Product Information

11549-1-AP targets CAMKK2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, monkey, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CAMKK2 fusion protein Ag2093
• Full Name: calcium/calmodulin-dependent protein kinase kinase 2, beta
• Calculated Molecular Weight: 588 aa, 60 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC026060
• Gene Symbol: CAMKK2
• Gene ID (NCBI): 10645
• RRID: AB_2259441
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96RR4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAMKK2(calcium/calmodulin-dependent protein kinase kinase 2) is also named as CAMKKB, KIAA0787 and belongs to the protein kinase superfamily. It regulates hypothalamic production of the orexigenic hormone NPY and contributes to the regulation of energy balance. CAMKK2 in vivo reduces tumor growth suggests that reactivation of CAMKK2 by development of new drugs may be a new strategy to prolong the period to respond androgen-deprivation therapy(ADT)(PMID:22549914). It can be autophosphorylated(PMID:19369195). It has 7 isoforms produced by alternative splicing.

Publications

Species	Application	Title
mouse	WB	Nature; Fructose-1,6-bisphosphate and aldolase mediate glucose sensing by AMPK.; Authors - Chen-Song Zhang
human	WB	Cancer Discov; Macropinocytosis in Cancer-Associated Fibroblasts Is Dependent on CaMKK2/ARHGEF2 Signaling and Functions to Support Tumor and Stromal Cell Fitness.; Authors - Yijuan Zhang ; KD Validated
human	WB	Cell Metab; CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress.; Authors - Qidong Li ; KD Validated
human	WB	Nat Commun; Cordycepin prevents radiation ulcer by inhibiting cell senescence via NRF2 and AMPK in rodents.; Authors - Ziwen Wang
human	WB	EMBO J; CARS senses cysteine deprivation to activate AMPK for cell survival.; Authors - Mengqiu Yuan ; KD Validated
mouse	WB,IF	Autophagy; m6A mRNA methylation regulates testosterone synthesis through modulating autophagy in Leydig cells.; Authors - Yabing Chen