CAP1 Polyclonal antibody

• 

  WB analysis using 16231-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16231-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16231-1-AP (CAP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 16231-1-AP

  IP result of anti-CAP1 (IP:16231-1-AP, 3ug; Detection:16231-1-AP 1:500) with A431 cells lysate 1520ug.

• 

  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 16231-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16231-1-AP (CAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

CAP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 16231-1-AP

Publications(5)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Adenylyl cyclase associated protein 1, Adenylyl cyclase-associated protein 1, CAP 1, CAP1 PEN

---

Tested Applications

• Positive WB detected in: Caco-2 cells, COLO 320 cells, HepG2 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IF: See 1 publications below

Product Information

16231-1-AP targets CAP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8876

  Product name: Recombinant human CAP1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 125-475 aa of BC013963

  Sequence: SKLFNHLSAVSESIQALGWVAMAPKPGPYVKEMNDAAMFYTNRVLKEYKDVDKKHVDWVKAYLSIWTELQAYIKEFHTTGLAWSKTGPVAKELSGLPSGPSAGSGPPPPPPGPPPPPVSTSSGSDESASRSALFAQINQGESITHALKHVSDDMKTHKNPALKAQSGPVRSGPKPFSAPKPQTSPSPKRATKKEPAVLELEGKKWRVENQENVSNLVIEDTELKQVAYIYKCVNTTLQIKGKINSITVDNCKKLGLVFDDVVGIVEIINSKDVKVQVMGKVPTISINKTDGCHAYLSKNSLDCEIVSAKSSEMNVLIPTEGGDFNEFPVPEQFKTLWNGQKLVTTVTEIAG

• Full Name: CAP, adenylate cyclase-associated protein 1 (yeast)
• Calculated Molecular Weight: 475 aa, 52 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC013963
• Gene Symbol: CAP1
• Gene ID (NCBI): 10487
• RRID: AB_2172166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q01518
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAP1, also known as CAP, CAP1-PEN, belongs to the CAP family and contains 1 C-CAP/cofactor C-like domain. CAP1 binds to monomeric actin and regulates the actin cytoskeleton to control cell size, adhesion, and migration (PMID: 23737525). CAP1 also functions as a receptor for inflammatory cytokine resistin (PMID: 24606903). CAP1 is an abundant and ubiquitously expressed protein. Western blot analysis detected CAP1 at an apparent molecular mass of 50-55 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Proc Natl Acad Sci U S A; CAP1 binds and activates adenylyl cyclase in mammalian cells.; Authors - Xuefeng Zhang
human	WB,IF	J Biol Chem; Cyclase-Associated Protein 1 (CAP1) is a prenyl-binding partner of Rap1 GTPase.; Authors - Xuefeng Zhang ; KD Validated
pig	WB	Viruses; Porcine Circovirus Type 2 Induces Single Immunoglobulin Interleukin-1 Related Receptor (SIGIRR) Downregulation to Promote Interleukin-1β Upregulation in Porcine Alveolar Macrophage.; Authors - Shunli Yang
bovine	WB	Cell Biochem Funct; Cyclase-associated protein 1 is a key negative regulator of milk synthesis and proliferation of bovine mammary epithelial cells.; Authors - Xiaohan Yuan ; KD Validated
mouse	WB	Cell Mol Life Sci; Functional interdependence of the actin regulators CAP1 and cofilin1 in control of dendritic spine morphology; Authors - Anika Heinze

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kuldeep (Verified Customer) (09-17-2025)

sharp band, little over 50KDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hippocamus homogenate

CAP1 Antibody Western Blot validation (1:1000 dilution) in Hippocamus homogenate (Cat no:16231-1-AP)