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CAPRIN1 Polyclonal antibody
CAPRIN1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 15112-1-AP
Validation Data Gallery
|Positive WB detected in
|HEK-293 cells, mouse brain tissue, HEK-293T cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
|Positive IP detected in
|Positive IHC detected in
|human breast cancer tissue, human prostate cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|HeLa cells, sodium arsenite treated HeLa cells, HepG2 cells, OC2 cells, cochlear cryosections
|Positive FC detected in
|Western Blot (WB)
|WB : 1:5000-1:50000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:200-1:800
|Flow Cytometry (FC)
|FC : 0.20 ug per 10^6 cells in a 100 µl suspension
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
15112-1-AP targets CAPRIN1 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|human, mouse, rat, zebrafish, pig
|Host / Isotype
|Rabbit / IgG
|CAPRIN1 fusion protein Ag7099
|cell cycle associated protein 1
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.
|Product Specific Protocols
|WB protocol for CAPRIN1 antibody 15112-1-AP
|IHC protocol for CAPRIN1 antibody 15112-1-AP
|IF protocol for CAPRIN1 antibody 15112-1-AP
|IP protocol for CAPRIN1 antibody 15112-1-AP
|Click here to view our Standard Protocols
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Katherine (Verified Customer) (10-31-2023)
The antibody worked well with 1:5000 dilution.
Lisa (Verified Customer) (02-20-2020)
The antibody works very well, giving specific bands in WB and a very good staining in IF.
Paul (Verified Customer) (04-30-2019)
Product works very well for WB, IP and IF using rat brain tissue samples.