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  • KD/KO Validated

CAPRIN1 Polyclonal antibody

CAPRIN1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 15112-1-AP


caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105

Tested Applications

Positive WB detected inHEK-293 cells, mouse brain tissue, HEK-293T cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, sodium arsenite treated HeLa cells, HepG2 cells, OC2 cells, cochlear cryosections
Positive FC detected inNIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15112-1-AP targets CAPRIN1 in WB, RIP, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPRIN1 fusion protein Ag7099
Full Name cell cycle associated protein 1
Calculated molecular weight 78 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC001731
Gene symbol CAPRIN1
Gene ID (NCBI) 4076
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.


Product Specific Protocols
WB protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IHC protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IF protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IP protocol for CAPRIN1 antibody 15112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Katherine (Verified Customer) (10-31-2023)

The antibody worked well with 1:5000 dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: 293T

Lisa (Verified Customer) (02-20-2020)

The antibody works very well, giving specific bands in WB and a very good staining in IF.

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: WB:1:2500 - IF:1:100
  • Cell Tissue Type: Fibroblasts, lymphoblastoid cell line
CAPRIN1 Antibody Western Blot,Immunofluorescence, validation (WB:1:2500 - IF:1:100 dilution) in Fibroblasts, lymphoblastoid cell line (Cat no:15112-1-AP)

Paul (Verified Customer) (04-30-2019)

Product works very well for WB, IP and IF using rat brain tissue samples.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Rat brain
CAPRIN1 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:2000 dilution) in Rat brain (Cat no:15112-1-AP)