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  • KD/KO Validated

CAPRIN1 Polyclonal antibody

CAPRIN1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15112-1-AP

Synonyms

caprin 1, CAPRIN1, GPI anchored protein p137, GPI p137, GPIAP1, GPIP137, M11S1, p137GPI, RNG105



Tested Applications

Positive WB detected inHeLa cells, HeLa and ND7 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, cochlear cryosections, Hela cells, OC2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15112-1-AP targets CAPRIN1 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CAPRIN1 fusion protein Ag7099
Full Name cell cycle associated protein 1
Calculated molecular weight 78 kDa
Observed molecular weight 116 kDa
GenBank accession numberBC001731
Gene symbol CAPRIN1
Gene ID (NCBI) 4076
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.

Protocols

Product Specific Protocols
WB protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IHC protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IF protocol for CAPRIN1 antibody 15112-1-APDownload protocol
IP protocol for CAPRIN1 antibody 15112-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Cell Mol Med

miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepatic stellate cells.

Authors - Baoling Ju
humanWB

Mol Cell

ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97.

Authors - Bo Wang
humanIP, WB

Biochim Biophys Acta

Characterization of O-GlcNAc cycling and proteomic identification of differentially O-GlcNAcylated proteins during G1/S transition.

Authors - Drougat Ludivine L
humanWB,IF

Int J Mol Sci

Arabidopsis thaliana G3BP Ortholog Rescues Mammalian Stress Granule Phenotype across Kingdoms.

Authors - Hendrik Reuper
mouseWB, IF

Dev Dyn

Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.

Authors - Soma Dash
  • KO Validated
humanWB

Sci Rep

Proteomic analysis of the SIRT6 interactome: novel links to genome maintenance and cellular stress signaling.

Authors - Simeoni Federica F
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Lisa (Verified Customer) (02-20-2020)

The antibody works very well, giving specific bands in WB and a very good staining in IF.

  • Applications: Western Blot,Immunofluorescence,
  • Primary Antibody Dilution: WB:1:2500 - IF:1:100
  • Cell Tissue Type: Fibroblasts, lymphoblastoid cell line
CAPRIN1 Antibody Western Blot,Immunofluorescence, validation (WB:1:2500 - IF:1:100 dilution) in Fibroblasts, lymphoblastoid cell line (Cat no:15112-1-AP)
FH

Paul (Verified Customer) (04-30-2019)

Product works very well for WB, IP and IF using rat brain tissue samples.

  • Applications: Western Blot,Immunofluorescence,Immunoprecipitation,
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Rat brain
CAPRIN1 Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:2000 dilution) in Rat brain (Cat no:15112-1-AP)