- Featured Product
- KD/KO Validated
CAPRIN1 Polyclonal antibody
CAPRIN1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 15112-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, HeLa and ND7 cells, mouse brain tissue, rat brain tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, human prostate cancer tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells, cochlear cryosections, Hela cells, OC2 cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
15112-1-AP targets CAPRIN1 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CAPRIN1 fusion protein Ag7099|
|Full Name||cell cycle associated protein 1|
|Calculated molecular weight||78 kDa|
|Observed molecular weight||116 kDa|
|GenBank accession number||BC001731|
|Gene ID (NCBI)||4076|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CAPRIN1 (Caprin-1) is a cytoplasmic phosphoprotein which may regulate the transport and translation of mRNAs of proteins involved in synaptic plasticity in neurons and cell proliferation and migration in multiple cell types. Its levels, except in the brain, are tightly correlated with cellular proliferation. Suppression of expression of human Caprin-1 resulted in slowing of the proliferation rate,which suggests its essential for normal cellular proliferation.
|Product Specific Protocols|
|WB protocol for CAPRIN1 antibody 15112-1-AP||Download protocol|
|IHC protocol for CAPRIN1 antibody 15112-1-AP||Download protocol|
|IF protocol for CAPRIN1 antibody 15112-1-AP||Download protocol|
|IP protocol for CAPRIN1 antibody 15112-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Cell Mol Med
miR-193a/b-3p relieves hepatic fibrosis and restrains proliferation and activation of hepatic stellate cells.
ULK1 and ULK2 Regulate Stress Granule Disassembly Through Phosphorylation and Activation of VCP/p97.
Biochim Biophys Acta
Characterization of O-GlcNAc cycling and proteomic identification of differentially O-GlcNAcylated proteins during G1/S transition.
Int J Mol Sci
Arabidopsis thaliana G3BP Ortholog Rescues Mammalian Stress Granule Phenotype across Kingdoms.
Int J Mol Sci
Mitochondrial Inhibition by Sodium Azide Induces Assembly of eIF2α Phosphorylation-Independent Stress Granules in Mammalian Cells.
Deficiency of the RNA binding protein Caprin2 causes lens defects and features of Peters anomaly.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Lisa (Verified Customer) (02-20-2020)
The antibody works very well, giving specific bands in WB and a very good staining in IF.
Paul (Verified Customer) (04-30-2019)
Product works very well for WB, IP and IF using rat brain tissue samples.