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Caspase 1/p20/p10 Polyclonal antibody
Caspase 1/p20/p10 Polyclonal Antibody for IF, IHC, WB, ELISA
Cat no : 22915-1-AP
|Positive WB detected in||RAW264.7, Jurkat cells, Raji cells, MCF-7 cells, HeLa cells, C6 cells, HepG2 cells, NIH/3T3 cells|
|Positive IHC detected in||human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||C6 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22915-1-AP is Caspase 1/p20/p10 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||bovine, human, monkey, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Caspase 1/p20/p10 fusion protein Ag11143|
|Full Name||caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)|
|Calculated molecular weight||404 aa, 45 kDa|
|Observed molecular weight||45-47 kDa, 30 kDa, 35 kDa|
|GenBank accession number||BC062327|
|Gene ID (NCBI)||834|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products(PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize all the five isoforms of caspase 1. It also can recognize p45 procaspase and the mature fragments p10 and p20.
|Product Specific Protocols|
|WB protocol for Caspase 1/p20/p10 antibody 22915-1-AP||Download protocol|
|IHC protocol for Caspase 1/p20/p10 antibody 22915-1-AP||Download protocol|
|IF protocol for Caspase 1/p20/p10 antibody 22915-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Joseph (Verified Customer) (02-19-2020)
This antibody detected pro-caspase 1 and the p20 cleaved product in PDAC cell lines. I did not look for the p10 cleaved product.
Tanusree (Verified Customer) (12-03-2019)
This antibody works good in western blotting analysis using mouse tissues.