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Caspase 1/p20/p10 Antibody 56 Publications

Rabbit Polyclonal| Catalog number: 22915-1-AP

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Con: 53 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Caspase 1/p20/p10 Antibody WB RAW264.7 22915-1-AP

RAW264.7 cells were subjected to SDS PAGE followed by western blot with 22915-1-AP(CASP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours

Caspase 1/p20/p10 Antibody IHC human spleen tissue 22915-1-AP

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP( Caspase 1 Antibody) at dilution of 1:200 (under 10x lens).

Caspase 1/p20/p10 Antibody IHC human spleen tissue 22915-1-AP

Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 22915-1-AP( Caspase 1 Antibody) at dilution of 1:200 (under 40x lens).

Caspase 1/p20/p10 Antibody IF C6 cells 22915-1-AP

Immunofluorescent analysis of C6 cells using 22915-1-AP(Caspase 1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse, rat, cow
Positive WB detected in:
RAW264.7, Raji cells, MCF-7 cells, NIH/3T3 cells, HeLa cells, C6 cells, Jurkat cells
Positive IHC detected in:
human spleen tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
C6 cells
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:100-1:400
IF : 1:10-1:100
Sample-dependent, check data in Validation tab

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Caspase 1/p20/p10 fusion protein Ag11143
Full name:
caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase)
Calculated molecular weight:
404aa,45 kDa
Observed molecular weight:
45-47 kDa,30 kDa,35 kDa
GenBank accession number:
BC062327
Gene ID (NCBI):
834
Gene symbol
CASP1
Synonyms
CASP 1, CASP1, Caspase 1, Caspase 1/p20/p10, Caspase1, ICE, IL 1 beta converting enzyme, IL 1BC, IL1BC, IL1BCE, Interleukin 1 beta convertase, p10, p20, P45

Background

CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products(PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize all the five isoforms of caspase 1. It also can recognize p45 procaspase and the mature fragments p10 and p20..


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