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Caspase 1/p20/p10 Polyclonal antibody
Caspase 1/p20/p10 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (4)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
424
Cat no : 22915-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | RAW264.7, Jurkat cells, Raji cells, MCF-7 cells, HeLa cells, C6 cells, HepG2 cells, NIH/3T3 cells |
Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | C6 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 386 publications below |
IHC | See 71 publications below |
IF | See 68 publications below |
Product Information
The immunogen of 22915-1-AP is Caspase 1/p20/p10 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, bovine, canine, monkey, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Caspase 1/p20/p10 fusion protein Ag11143 |
Full Name | caspase 1, apoptosis-related cysteine peptidase (interleukin 1, beta, convertase) |
Calculated molecular weight | 404 aa, 45 kDa |
Observed molecular weight | 45-47 kDa, 30 kDa, 35 kDa |
GenBank accession number | BC062327 |
Gene symbol | CASP1 |
Gene ID (NCBI) | 834 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). It has 5 isoforms produced by alternative splicing. 22915-1-AP can recognize all the five isoforms of caspase 1. It also can recognize p45 procaspase and the mature fragments p10 and p20.
Protocols
Product Specific Protocols | |
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WB protocol for Caspase 1/p20/p10 antibody 22915-1-AP | Download protocol |
IHC protocol for Caspase 1/p20/p10 antibody 22915-1-AP | Download protocol |
IF protocol for Caspase 1/p20/p10 antibody 22915-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun REV-ERBα integrates colon clock with experimental colitis through regulation of NF-κB/NLRP3 axis. | ||
J Clin Invest Melatonin inhibits cytosolic mitochondrial DNA-induced neuroinflammatory signaling in accelerated aging and neurodegeneration. | ||
Theranostics Ablation of NLRP3 inflammasome attenuates muscle atrophy via inhibiting pyroptosis, proteolysis and apoptosis following denervation | ||
J Neuroinflammation NLRP3 deficiency decreases alcohol intake controlling anxiety-like behavior via modification of glutamatergic transmission in corticostriatal circuits | ||
Ann Neurol Subicular Caspase-1 Contributes to Pharmacoresistance in Temporal Lobe Epilepsy. | ||
Cell Mol Immunol NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Joseph (Verified Customer) (02-19-2020) | This antibody detected pro-caspase 1 and the p20 cleaved product in PDAC cell lines. I did not look for the p10 cleaved product.
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FH Tanusree (Verified Customer) (12-03-2019) | This antibody works good in western blotting analysis using mouse tissues.
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