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Caspase 3/p17/p19 Monoclonal antibody
Caspase 3/p17/p19 Monoclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (4)
Applications
WB, IHC, IF, ICC, ELISA
Conjugate
Unconjugated
113
CloneNo.
2G4B2
Cat no : 66470-2-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, NIH/3T3 cells, HepG2 cells, HSC-T6 cells, HEK-293 cells |
Positive IHC detected in | human breast cancer tissue, mouse liver tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human breast cancer tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 102 publications below |
IHC | See 10 publications below |
IF | See 5 publications below |
Product Information
66470-2-Ig targets Caspase 3/p17/p19 in WB, IHC, IF, ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Cited Reactivity | human, mouse, rat, canine, chicken, plant |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Caspase 3/p17/p19 fusion protein Ag25029 |
Full Name | caspase 3, apoptosis-related cysteine peptidase |
Calculated molecular weight | 277 aa, 32 kDa |
Observed molecular weight | 32-35 kDa, 19 kDa, 17 kDa |
GenBank accession number | BC016926 |
Gene symbol | CASP3 |
Gene ID (NCBI) | 836 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Caspase 3/p17/p19 antibody 66470-2-Ig | Download protocol |
IHC protocol for Caspase 3/p17/p19 antibody 66470-2-Ig | Download protocol |
IF protocol for Caspase 3/p17/p19 antibody 66470-2-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Brain Behav Immun Extracellular RNAs-TLR3 signaling contributes to cognitive decline in a mouse model of postoperative cognitive dysfunction. | ||
Life Sci Melatonin protects circulatory death heart from ischemia/reperfusion injury via the JAK2/STAT3 signalling pathway. | ||
Int J Biochem Cell Biol Knockdown of LncRNA MALAT1 contributes to cell apoptosis via regulating NF-κB/CD80 axis in neonatal respiratory distress syndrome. | ||
Nan Fang Yi Ke Da Xue Xue Bao [JNK/c-Jun signaling pathway mediates arginine vasopressin neuron regeneration by promoting cytoskeleton reconstruction in rats with electrical lesions of the pituitary stalk]. | ||
Cancers (Basel) A Splice Variant of NCOR2, BQ323636.1, Confers Chemoresistance in Breast Cancer by Altering the Activity of NRF2. | ||
Cell Rep CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression. |