- Featured Product
- KD/KO Validated
Caspase 3/p17/p19 Monoclonal antibody
Caspase 3/p17/p19 Monoclonal Antibody for IF, IHC, WB,ELISA
Cat no : 66470-2-Ig
|Positive WB detected in||Jurkat cells, NIH/3T3 cells, HepG2 cells, HSC-T6 cells, HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue, mouse liver tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue|
|Western Blot (WB)||WB : 1:1000-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:150-1:600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66470-2-Ig targets Caspase 3/p17/p19 in WB, IHC, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||canine, human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Caspase 3/p17/p19 fusion protein Ag25029|
|Full Name||caspase 3, apoptosis-related cysteine peptidase|
|Calculated molecular weight||277 aa, 32 kDa|
|Observed molecular weight||32-35 kDa, 19 kDa, 17 kDa|
|GenBank accession number||BC016926|
|Gene ID (NCBI)||836|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
|Product Specific Protocols|
|WB protocol for Caspase 3/p17/p19 antibody 66470-2-Ig||Download protocol|
|IHC protocol for Caspase 3/p17/p19 antibody 66470-2-Ig||Download protocol|
|IF protocol for Caspase 3/p17/p19 antibody 66470-2-Ig||Download protocol|
|Click here to view our Standard Protocols|
Brain Behav Immun
Extracellular RNAs-TLR3 signaling contributes to cognitive decline in a mouse model of postoperative cognitive dysfunction.
Melatonin protects circulatory death heart from ischemia/reperfusion injury via the JAK2/STAT3 signalling pathway.
Int J Biochem Cell Biol
Knockdown of LncRNA MALAT1 contributes to cell apoptosis via regulating NF-κB/CD80 axis in neonatal respiratory distress syndrome.
Nan Fang Yi Ke Da Xue Xue Bao
[JNK/c-Jun signaling pathway mediates arginine vasopressin neuron regeneration by promoting cytoskeleton reconstruction in rats with electrical lesions of the pituitary stalk].
A Splice Variant of NCOR2, BQ323636.1, Confers Chemoresistance in Breast Cancer by Altering the Activity of NRF2.
CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.