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Caspase 3/p17/p19 Antibody
Caspase 3/p17/p19 Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66470-2-Ig
Apopain, CASP 3, CASP3, Caspase 3, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
|Positive WB detected in||Jurkat cells, NIH/3T3 cells, HepG2 cells, HSC-T6 cells, HEK-293 cells|
|Positive IHC detected in||human breast cancer tissue, mouse liver tissue, mouse kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue|
|Western Blot (WB)||WB : 1:1000-1:3000|
|Immunohistochemistry (IHC)||IHC : 1:150-1:600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66470-2-Ig targets Caspase 3/p17/p19 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
|Tested Reactivity||Human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Caspase 3/p17/p19 fusion protein Ag25029|
|Full Name||caspase 3, apoptosis-related cysteine peptidase|
|Calculated molecular weight||277aa,32 kDa|
|Observed molecular weight||32-35 kDa,19 kDa,17 kDa|
|GenBank accession number||BC016926|
|Gene ID (NCBI)||836|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
|Product Specific Protocols|
|WB protocol for Caspase 3/p17/p19 antibody 66470-2-Ig||Download protocol|
|IHC protocol for Caspase 3/p17/p19 antibody 66470-2-Ig||Download protocol|
|IF protocol for Caspase 3/p17/p19 antibody 66470-2-Ig||Download protocol|
|Click here to view our Standard Protocols|
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