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Caspase 3/p17/p19 Monoclonal antibody

Caspase 3/p17/p19 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2G4B2

Cat no : 66470-2-Ig

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1



Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells, HepG2 cells, HSC-T6 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue, mouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66470-2-Ig targets Caspase 3/p17/p19 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Caspase 3/p17/p19 fusion protein Ag25029
Full Name caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight 277 aa, 32 kDa
Observed molecular weight 32-35 kDa, 19 kDa, 17 kDa
GenBank accession numberBC016926
Gene symbol CASP3
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Protocols

Product Specific Protocols
WB protocol for Caspase 3/p17/p19 antibody 66470-2-IgDownload protocol
IHC protocol for Caspase 3/p17/p19 antibody 66470-2-IgDownload protocol
IF protocol for Caspase 3/p17/p19 antibody 66470-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Brain Behav Immun

Extracellular RNAs-TLR3 signaling contributes to cognitive decline in a mouse model of postoperative cognitive dysfunction.

Authors - Chan Chen
ratWB

Life Sci

Melatonin protects circulatory death heart from ischemia/reperfusion injury via the JAK2/STAT3 signalling pathway.

Authors - Hongwen Lan
humanWB

Int J Biochem Cell Biol

Knockdown of LncRNA MALAT1 contributes to cell apoptosis via regulating NF-κB/CD80 axis in neonatal respiratory distress syndrome.

Authors - Chenxia Juan
ratIF

Nan Fang Yi Ke Da Xue Xue Bao

[JNK/c-Jun signaling pathway mediates arginine vasopressin neuron regeneration by promoting cytoskeleton reconstruction in rats with electrical lesions of the pituitary stalk].

Authors - Kai Li
humanWB

Cancers (Basel)

A Splice Variant of NCOR2, BQ323636.1, Confers Chemoresistance in Breast Cancer by Altering the Activity of NRF2.

Authors - Man-Hong Leung
humanWB

Cell Rep

CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.

Authors - H Kubra Gurkaslar