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Caspase 3/p17/p19 Monoclonal antibody

Caspase 3/p17/p19 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1


Human, mouse, rat and More (4)







Cat no : 66470-2-Ig


Apopain, CASP 3, CASP3, Caspase 3, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells, HepG2 cells, HSC-T6 cells, HEK-293 cells
Positive IHC detected inhuman breast cancer tissue, mouse liver tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66470-2-Ig targets Caspase 3/p17/p19 in WB, IHC, IF, ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, chicken, plant
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Caspase 3/p17/p19 fusion protein Ag25029
Full Name caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight 277 aa, 32 kDa
Observed molecular weight 32-35 kDa, 19 kDa, 17 kDa
GenBank accession numberBC016926
Gene symbol CASP3
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.


Product Specific Protocols
WB protocol for Caspase 3/p17/p19 antibody 66470-2-IgDownload protocol
IHC protocol for Caspase 3/p17/p19 antibody 66470-2-IgDownload protocol
IF protocol for Caspase 3/p17/p19 antibody 66470-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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