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Caspase 3 Antibody 5 Publications

Mouse Monoclonal| Catalog number: 66470-2-Ig |CloneNo.: 2G4B2

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Con: 150 μg/150 μl

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Tested Applications

Applications:
WB, IHC, IF, ELISA
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Images See all

Caspase 3 Antibody WB HEK-293 cells 66470-2-Ig

WB result of Caspase 3 antibody (66470-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected HEK-293 cells

Caspase 3 Antibody WB Jurkat cells 66470-2-Ig

Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours

Caspase 3 Antibody IHC human breast cancer tissue 66470-2-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig( CASP3 antibody) at dilution of 1:300 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Caspase 3 Antibody IHC human breast cancer tissue 66470-2-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig( CASP3 antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Caspase 3 Antibody IF human breast cancer tissue 66470-2-Ig

Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 66470-2-Ig (CASP3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
Human, mouse, rat
Positive WB detected in:
Jurkat cells, NIH/3T3 cells, HepG2 cells, HSC-T6 cells, HEK-293 cells
Positive IHC detected in:
human breast cancer tissue, mouse liver tissue, mouse kidney tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human breast cancer tissue
Recommended dilution:
WB : 1:1000-1:6000
IHC : 1:150-1:600
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Caspase 3 fusion protein Ag25029
Full name:
caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight:
277aa,32 kDa
Observed molecular weight:
32-35 kDa,19 kDa,17 kDa
GenBank accession number:
BC016926
Gene ID (NCBI):
836
Gene symbol
CASP3
Synonyms
Apopain, CASP 3, CASP3, Caspase 3, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

Background

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.


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