Caspase 3/p17/p19 Monoclonal antibody

• Featured Product
• KD/KO Validated

Caspase 3/p17/p19 Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (4)

Applications

WB, IHC, IF, ICC, ELISA

Conjugate

Unconjugated

Publications

113

CloneNo.

2G4B2

Cat no : 66470-2-Ig

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 66470-2-Ig

  WB result of Caspase 3 antibody (66470-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected HEK-293 cells.

• 

  WB analysis of Jurkat using 66470-2-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66470-2-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66470-2-Ig (Caspase 3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66470-2-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 66470-2-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66470-2-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66470-2-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 66470-2-Ig

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 66470-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 66470-2-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66470-2-Ig (CASP3 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: Jurkat cells, NIH/3T3 cells, HepG2 cells, HSC-T6 cells, HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, mouse liver tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human breast cancer tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 102 publications below
• IHC: See 10 publications below
• IF: See 5 publications below

Product Information

66470-2-Ig targets Caspase 3/p17/p19 in WB, IHC, IF, ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, chicken, plant
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 3/p17/p19 fusion protein Ag25029
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated molecular weight: 277 aa, 32 kDa
• Observed molecular weight: 32-35 kDa, 19 kDa, 17 kDa
• GenBank accession number: BC016926
• Gene symbol: CASP3
• Gene ID (NCBI): 836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

Publications

Species	Application	Title
mouse	WB	Brain Behav Immun; Extracellular RNAs-TLR3 signaling contributes to cognitive decline in a mouse model of postoperative cognitive dysfunction.; Authors - Chan Chen
rat	WB	Life Sci; Melatonin protects circulatory death heart from ischemia/reperfusion injury via the JAK2/STAT3 signalling pathway.; Authors - Hongwen Lan
human	WB	Int J Biochem Cell Biol; Knockdown of LncRNA MALAT1 contributes to cell apoptosis via regulating NF-κB/CD80 axis in neonatal respiratory distress syndrome.; Authors - Chenxia Juan
rat	IF	Nan Fang Yi Ke Da Xue Xue Bao; [JNK/c-Jun signaling pathway mediates arginine vasopressin neuron regeneration by promoting cytoskeleton reconstruction in rats with electrical lesions of the pituitary stalk].; Authors - Kai Li
human	WB	Cancers (Basel); A Splice Variant of NCOR2, BQ323636.1, Confers Chemoresistance in Breast Cancer by Altering the Activity of NRF2.; Authors - Man-Hong Leung
human	WB	Cell Rep; CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression.; Authors - H Kubra Gurkaslar