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CASP3 Antibody 2 Publications

Mouse Monoclonal| Catalog number: 66470-2-Ig |CloneNo.: 2G4B2

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Tested Applications
Applications:
ELISA, IF, IHC, WB
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CASP3 Antibody WB Jurkat cells 66470-2-Ig

Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours

CASP3 Antibody IHC human breast cancer tissue 66470-2-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig( CASP3 antibody) at dilution of 1:300 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CASP3 Antibody IHC human breast cancer tissue 66470-2-Ig

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig( CASP3 antibody) at dilution of 1:300 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

CASP3 Antibody IF human breast cancer tissue 66470-2-Ig

Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 66470-2-Ig (CASP3 antibody) at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

Species specificity:
Human, mouse, rat
Positive WB detected in:
Jurkat cells,HEK-293 cells, HSCT6 cells, NIH/3T3 cells
Positive IHC detected in:
human breast cancer tissue
Positive IF detected in:
human breast cancer tissue
Recommended dilution:
WB : 1:500-1:2000
IHC : 1:150-1:600
IF : 1:50-1:500

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
CASP3 fusion protein Ag25029
Full name:
caspase 3, apoptosis-related cysteine peptidase
Calculated molecular weight:
277aa,32 kDa
Observed molecular weight:
32-35 kDa,19 kDa,17 kDa
GenBank accession number:
BC016926
Gene ID (NCBI):
836
Gene symbol
CASP3
Synonyms
Apopain, CASP 3, CASP3, Caspase 3, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
Background

Casp3 (caspase 3), also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. CASP3 is involved in the cleavage of huntingtin. This antibody can recognize the 32 kDa pro-caspase 3 as well as 17 and 19 kDa cleaved-caspase 3.


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