Caspase 3/p17/p19 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Caspase 3/p17/p19 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G4B2

Cat no : 66470-2-PBS

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17/p19, Caspase3, Caspase-3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

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Validation Data Gallery

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  WB analysis of HEK-293 using 66470-2-Ig (same clone as 66470-2-PBS)

  WB result of Caspase 3 antibody (66470-2-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Caspase 3 transfected HEK-293 cells. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66470-2-Ig (same clone as 66470-2-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 66470-2-Ig (same clone as 66470-2-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66470-2-Ig (same clone as 66470-2-PBS)

  Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 66470-2-Ig (CASP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  WB analysis using 66470-2-Ig (same clone as 66470-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66470-2-Ig (Caspase 3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66470-2-Ig (CASP3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 66470-2-Ig (same clone as 66470-2-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66470-2-Ig (Caspase 3 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66470-2-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66470-2-PBS targets Caspase 3/p17/p19 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Caspase 3/p17/p19 fusion protein Ag25029
• Full Name: caspase 3, apoptosis-related cysteine peptidase
• Calculated Molecular Weight: 277 aa, 32 kDa
• Observed Molecular Weight: 32-35 kDa, 19 kDa, 17 kDa
• GenBank Accession Number: BC016926
• Gene Symbol: CASP3
• Gene ID (NCBI): 836
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.