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  • KD/KO Validated

Caspase 4/p20/p10 Polyclonal antibody

Caspase 4/p20/p10 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11856-1-AP

Synonyms

CASP 4, CASP4, Caspase 4, Caspase 4/p20/p10, Caspase4, ICE(rel) II, ICE(rel)II, ICEREL II, ICH 2, ICH2, Mih1/TX, Protease ICH 2, Protease TX, TX



Tested Applications

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11856-1-AP targets Caspase 4/p20/p10 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 4/p20/p10 fusion protein Ag2429
Full Name caspase 4, apoptosis-related cysteine peptidase
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight 43-48 kDa
GenBank accession numberBC017839
Gene symbol CASP4
Gene ID (NCBI) 837
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

Protocols

Product Specific Protocols
WB protocol for Caspase 4/p20/p10 antibody 11856-1-APDownload protocol
IHC protocol for Caspase 4/p20/p10 antibody 11856-1-APDownload protocol
IF protocol for Caspase 4/p20/p10 antibody 11856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,WB

Front Immunol

Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma

Authors - Liangmin Fu
  • KD Validated
humanWB

Front Microbiol

Enterovirus 71 Induces INF2 Cleavage via Activated Caspase-2 in Infected RD Cells.

Authors - Bei Wang
humanWB

Front Microbiol

Porphyromonas gingivalis-Induced NLRP3 Inflammasome Activation and Its Downstream Interleukin-1β Release Depend on Caspase-4.

Authors - Pei-Hui Ding
humanWB

Chem Res Toxicol

Atypical Gasdermin D and Mixed Lineage Kinase Domain-like Protein Leakage Aggravates Tetrachlorobenzoquinone-Induced Nod-like Receptor Protein 3 Inflammasome Activation.

Authors - Xiaomin Xia
humanWB

Int J Mol Sci

PMID: 27827850

Authors - Wei-Ye Shi
humanWB

Inflammation

Aspergillus fumigatus-Stimulated Human Corneal Epithelial Cells Induce Pyroptosis of THP-1 Macrophages by Secreting TSLP.

Authors - Qingshan Ji