Caspase 4/p20/p10 Polyclonal antibody

Caspase 4/p20/p10 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11856-1-AP

Synonyms

CASP 4, CASP4, Caspase 4, Caspase4, ICE(rel) II, ICE(rel)II, ICEREL II, ICH 2, ICH2, Mih1/TX, Protease ICH 2, Protease TX, TX



Tested Applications

Positive WB detected inSGC-7901 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11856-1-AP targets Caspase 4/p20/p10 in WB, IHC, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caspase 4/p20/p10 fusion protein Ag2429
Full Name caspase 4, apoptosis-related cysteine peptidase
Calculated molecular weight 377 aa, 43 kDa
Observed molecular weight 43-48 kDa
GenBank accession numberBC017839
Gene symbol CASP4
Gene ID (NCBI) 837
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.

Protocols

Product Specific Protocols
WB protocol for Caspase 4/p20/p10 antibody 11856-1-APDownload protocol
IHC protocol for Caspase 4/p20/p10 antibody 11856-1-APDownload protocol
IF protocol for Caspase 4/p20/p10 antibody 11856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Med Sci Monit

Mechanisms of IFNalpha-1a-Induced Apoptosis in a Laryngeal Cancer Cell Line.

Authors - Xiao-Lei Xin
humanWB

Stem Cells Int

The Role of Caspase-4 and NLRP1 in MCF7 Cell Pyroptosis Induced by hUCMSC-Secreted Factors.

Authors - Yang Jiao
  • KD Validated
humanWB

Exp Ther Med

IGF2BP2 knockdown inhibits LPS-induced pyroptosis in BEAS-2B cells by targeting caspase 4, a crucial molecule of the non-canonical pyroptosis pathway.

Authors - Jing Wang
humanWB

Inflammation

Aspergillus fumigatus-Stimulated Human Corneal Epithelial Cells Induce Pyroptosis of THP-1 Macrophages by Secreting TSLP.

Authors - Qingshan Ji
humanWB

Int J Mol Sci

PMID: 27827850

Authors - Wei-Ye Shi
humanWB

Chem Res Toxicol

Atypical Gasdermin D and Mixed Lineage Kinase Domain-like Protein Leakage Aggravates Tetrachlorobenzoquinone-Induced Nod-like Receptor Protein 3 Inflammasome Activation.

Authors - Xiaomin Xia