Tested Applications
| Positive WB detected in | SGC-7901 cells, HepG2 cells, THP-1 cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11856-1-AP targets Caspase 4/P20/P10 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2429 Product name: Recombinant human Caspase 4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-377 aa of BC017839 Sequence: MAEGNHRKKPLKVLESLGKDFLTGVLDNLVEQNVLNWKEEEKKKYYDAKTEDKVRVMADSMQEKQRMAGQMLLQTFFNIDQISPNKKAHPNMEAGPPESGESTDALKLCPHEEFLRLCKERAEEIYPIKERNNRTRLALIICNTEFDHLPPRNGADFDITGMKELLEGLDYSVDVEENLTARDMESALRAFATRPEHKSSDSTFLVLMSHGILEGICGTVHDEKKPDVLLYDTIFQIFNNRNCLSLKDKPKVIIVQACRGANRGELWVRDSPASLEVASSQSSENLEEDAVYKTHVEKDFIAFCSSTPHNVSWRDSTMGSIFITQLITCFQKYSWCCHLEEVFRKVQQSFETPRAKAQMPTIERLSMTRYFYLFPGN Predict reactive species |
| Full Name | caspase 4, apoptosis-related cysteine peptidase |
| Calculated Molecular Weight | 377 aa, 43 kDa |
| Observed Molecular Weight | 43-48 kDa |
| GenBank Accession Number | BC017839 |
| Gene Symbol | Caspase 4 |
| Gene ID (NCBI) | 837 |
| RRID | AB_2070053 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P49662 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Caspase 4 (CASP4), is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.Caspase 4 is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. Overexpression of caspase 4 will induce cell apoptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Caspase 4/P20/P10 antibody 11856-1-AP | Download protocol |
| IHC protocol for Caspase 4/P20/P10 antibody 11856-1-AP | Download protocol |
| WB protocol for Caspase 4/P20/P10 antibody 11856-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Caspase 4/P20/P10 Polyclonal antibody (Cat# 11856-1-AP) has 25 citations across journals including Theranostics, Research, Phytomedicine, Frontiers in Immunology, Frontiers in Microbiology, Journal of Ethnopharmacology, Annals of Medicine, Biomedicines, and Stem Cells International. Research fields span pyroptosis, inflammasome activation, cancer (colorectal, renal, glioma, pancreatic), diabetic kidney disease, neuroinflammation, psoriasis, and infectious diseases.
| Species | Application | Title |
|---|---|---|
Theranostics Outer membrane vesicles derived from gut microbiota mediate tubulointerstitial inflammation: a potential new mechanism for diabetic kidney disease
| ||
Research (Wash D C) IGF2BP1/HMOX1 Mediates Fate Determinations of Human Spermatogonial Stem Cells and Male Infertility via an m6A-Dependent Manner. | ||
Phytomedicine Regulation of NLRP3 inflammasome and Caspase-3/4/11 by 2',4'-dihydroxychalcone contributes to anti-colorectal cancer | ||
Microbiol Res Caspase-4/11-mediated non-canonical pyroptosis disrupts blood-brain barrier integrity via VEGFA/VEGFR2 signaling in Escherichia coli meningitis. | ||
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma
| ||
J Ethnopharmacol Aurantii Fructus alleviates depression via modulating hippocampal caspase-4-linked neurogenesis and synaptic plasticity in mice. |
Reviews
What customers say
The single review for this product is highly positive, rated 5 out of 5 stars. The reviewer used the antibody for Western Blot at a 1:1000 dilution on hepatocytes and reported that it works well, producing reliable results. Overall, the feedback indicates strong performance and satisfaction with the antibody's functionality in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Shan (Verified Customer) (07-10-2025) | The antibody works good when tested by western blot.
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