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Caveolin-1 Antibody

Caveolin-1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16447-1-AP

Synonyms

BSCL3, CAV, CAV1, Caveolin 1, Caveolin-1, CGL3, MSTP085, VIP21



Tested Applications

Positive WB detected inNIH/3T3 cells, A549 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue, human heart tissue, human liver tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:600-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16447-1-AP is Caveolin-1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Caveolin-1 fusion protein Ag8049
Full Name caveolin 1, caveolae protein, 22kDa
Calculated molecular weight 22 kDa
Observed molecular weight 20-25 kDa
GenBank accession numberBC006432
Gene symbol CAV1
Gene ID (NCBI) 857
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Caveolin-1 (CAV1), a multifunctional protein, is the main constituent molecule of caveolae and represents a scaffolding molecule for several signaling molecules including epidermal growth factor receptor (PMID: 19641024). Several studies have implicated that a reduced expression of CAV1 was found in cancers including head and neck carcinoma (PMID: 19002186). However, other studies recognize CAV1 as a tumor promoter because CAV1 is overexpressed in various kinds of cancers, especially in oral cancer (PMID: 20558341). Recent study also show that CAV1 is involved in astric Cancer (PMID: 25339030). MW of Caveolin-1 is from 20-25 kDa due to phosphorylation (PMID: 10198051).

Protocols

Product Specific Protocols
WB protocol for Caveolin-1 antibody 16447-1-APDownload protocol
IHC protocol for Caveolin-1 antibody 16447-1-APDownload protocol
IP protocol for Caveolin-1 antibody 16447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IHC

Am J Physiol Lung Cell Mol Physiol

Identification of CAV1 and DCN as potential predictive biomarkers for lung adenocarcinoma.

Authors - Yuanliang Yan
ratWB

Am J Chin Med

Puerarin Reduces Blood Pressure in Spontaneously Hypertensive Rats by Targeting eNOS.

Authors - Weili Shi
humanWB,IHC

Biochem Pharmacol

Betulinic acid suppresses breast cancer aerobic glycolysis via caveolin-1/NF-κB/c-Myc pathway.

Authors - Lin Jiao
humanWB,IHC

FASEB J

Tumoral microvesicle-activated glycometabolic reprogramming in fibroblasts promotes the progression of oral squamous cell carcinoma.

Authors - Erhui Jiang
  • KD Validated
humanWB

Am J Respir Crit Care Med

Distinct Cancer-Promoting Stromal Gene Expression Depending on Lung Function.

Authors - Brian J Sandri
humanWB

EBioMedicine

POH1 contributes to hyperactivation of TGF-β signaling and facilitates hepatocellular carcinoma metastasis through deubiquitinating TGF-β receptors and caveolin-1.

Authors - Boshi Wang