Anti-Mouse CCL20/MIP-3 alpha Rabbit Recombinant Antibody

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  FC experiment of Transfected HEK-293T using 98519-1-RR

  1x10^6 HEK-293T cells or CCL20/MIP-3 alpha transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL20/MIP-3 alpha Rabbit RecAb (98519-1-RR, Clone: 250239E12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Transfected HEK-293T using 98519-1-RR

  1x10^6 HEK-293T cells or CCL20/MIP-3 alpha-transfected HEK-293T cells were intracellularly stained with 0.25 ug Anti-Mouse CCL20/MIP-3 alpha Rabbit RecAb (98519-1-RR, Clone: 250239E12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CCL20/MIP-3 alpha Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98519-1-RR

Clone No.250239E12

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

Beta-chemokine exodus-1, CC chemokine LARC, CC chemokine ST38, C-C motif chemokine 20, CCL 20

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Tested Applications

• Positive FC (Intra) detected in: Transfected HEK-293T cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98519-1-RR targets CCL20/MIP-3 alpha in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CCL20/MIP-3 alpha fusion protein Eg3311
• Full Name: chemokine (C-C motif) ligand 20
• Calculated Molecular Weight: 11KD
• GenBank Accession Number: NM_001159738.1
• Gene Symbol: Ccl20
• Gene ID (NCBI): 20297
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q642U4
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CCL20, also known as macrophage infiltrating factor protein 3α, is a C-C chemokine that specifically binds to the receptor CCR6. CCL20 is produced in various tissues and by a wide range of immune cells, including neutrophils, natural killer cells, T-helper type 17 (Th17) cells, B cells, and various antigen-presenting cells, such as dendritic cells (DCs), Langerhans cells, and macrophages. Increased expression of CCL20 is likely involved in the increased recruitment of dendritic cells observed in fibroinflammatory diseases such as chronic obstructive pulmonary disease (COPD). CCL20 expression is increased by the proinflammatory cytokine IL-1 beta.