CCL3/MIP-1 alpha Polyclonal antibody

CCL3/MIP-1 alpha Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32190-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

C-C motif chemokine 3, Ccl3, Heparin-binding chemotaxis protein, L2G25B, Macrophage inflammatory protein 1-alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 400 ug/ml
20 μL, 400 ug/ml
150 μL, 400 ug/ml

$449/ 150 μL

In stock in Rosemont, IL. Order within 12 hours and 42 mins to receive by Thursday, July 10

Freight/Packing: $40.00

Quantity

Tested Applications

Positive WB detected inLPS and Brefeldin A treated RAW 264.7 cells
Positive IF/ICC detected inPMA, LPS and Brefeldin A treated THP-1 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32190-1-AP targets CCL3/MIP-1 alpha in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CCL3/MIP-1 alpha fusion protein Eg2170 Predict reactive species
Full Name chemokine (C-C motif) ligand 3
Calculated Molecular Weight10kd
Observed Molecular Weight11 kDa
GenBank Accession NumberNM_011337
Gene Symbol Ccl3
Gene ID (NCBI) 20302
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDP10855
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CCL3/MIP-1 alpha antibody 32190-1-APDownload protocol
IF protocol for CCL3/MIP-1 alpha antibody 32190-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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