Anti-Mouse CCL9 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of Transfected HEK-293T using 98475-1-RR (same clone as 98475-1-PBS)

  1x10^6 HEK-293T cells (left) or CCL9-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL9 Rabbit RecAb (98475-1-RR, Clone: 243103H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98475-1-PBS in a different storage buffer formulation.

• 

  FC experiment of Transfected HEK-293T using 98475-1-RR (same clone as 98475-1-PBS)

  1x10^6 HEK-293T cells (left) or CCL9-transfected HEK-293T cells (right) were intracellularly stained with 0.25 ug Anti-Mouse CCL9 Rabbit RecAb (98475-1-RR, Clone:243103H5) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98475-1-PBS in a different storage buffer formulation.

CCL9 Uni-rAb^TM Recombinant Antibody for FC (Intra)

Cat No. 98475-1-PBS

Clone No.243103H5

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Synonyms

C-C motif chemokine 9, CCF18, CCL9(29-101), CCL9(30-101), CCL9(31-101)

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Product Information

98475-1-PBS targets CCL9 in FC (Intra) applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CCL9 fusion protein Eg3336
• Full Name: chemokine (C-C motif) ligand 9
• Calculated Molecular Weight: 14KD
• GenBank Accession Number: NM_011338.2
• Gene Symbol: Ccl9
• Gene ID (NCBI): 20308
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q3U9T8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Mouse C-C motif ligand 9 (CCL9), alternatively named macrophage inflammatory protein 1γ (MIP-1γ), was identified in 1995 and is homologous to the mouse CCL6, as well as human CCL23 and CCL15. It is noted that CCL9 is also known by various names such as macrophage inflammatory protein-1 gamma (MIP-1ɣ), macrophage inflammatory protein-related protein-2 (MRP-2) and CCF18 in rodents. Monocytes and myeloid cell lines produce large quantities of CCL9, as do dendritic cells and T cells, in particular Th1 type T cells. Despite high baseline levels in circulation, it has become apparent that concentrations of CCL9 vary greatly in specific tissues with profound effects on health. For example, in the bone, CCL9 is produced at even higher levels, and is critical to osteoclast versus osteoblast differentiation of macrophages. There are also indications of a timed specific induction of CCL9 in skin wound healing and follicle-associated epithelium of the gut.