Tested Applications
| Positive WB detected in | Jurkat cells, mouse skeletal muscle tissue, rat skeletal muscle tissue |
| Positive IHC detected in | mouse kidney tissue, human kidney tissue, human ovary tumor tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Jurkat cells, THP-1 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16153-1-AP targets CCR2a-specific in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | chemokine (C-C motif) receptor 2 |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 36-42 kDa |
| GenBank Accession Number | NM_000647 |
| Gene Symbol | CCR2 |
| Gene ID (NCBI) | 729230 |
| RRID | AB_2262945 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41597 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CCR2a-specific antibody 16153-1-AP | Download protocol |
| IF protocol for CCR2a-specific antibody 16153-1-AP | Download protocol |
| IHC protocol for CCR2a-specific antibody 16153-1-AP | Download protocol |
| WB protocol for CCR2a-specific antibody 16153-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CCR2a-specific Polyclonal antibody (Cat# 16153-1-AP) has 53 citations. These appear across high-impact journals including Nature Communications, Neuron, Advanced Materials, Advanced Science, Cell Reports, Cell Death & Differentiation, Biomaterials, Theranostics, Acta Pharmaceutica Sinica B, and Frontiers journals. Research fields span immunology (macrophage/monocyte biology, CCL2-CCR2 axis), oncology (tumor-associated macrophages, metastasis), neurology (stroke, neuropathic pain), cardiology, nephrology, fibrosis, and inflammatory disease. Applications include WB, IF, IHC, and IP.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Camouflaging attenuated Salmonella by cryo-shocked macrophages for tumor-targeted therapy | ||
Bone Res Single-cell sequencing reveals a senescent immune landscape in bone marrow lesions inducing articular cartilage damage in osteoarthritis. | ||
Nat Commun Engineered Spirulina platensis for treating rheumatoid arthritis and restoring bone homeostasis | ||
Neuron Macrophage-derived CTSS drives the age-dependent disruption of the blood-CSF barrier | ||
J Nanobiotechnology Engineered neutrophil membrane-camouflaged nanocomplexes for targeted siRNA delivery against myocardial ischemia reperfusion injury |
Reviews
What customers say
All three reviews rate the antibody 5 stars. Users report excellent performance in immunofluorescence on mouse bone marrow mesenchymal stem cells (1:200) and paraffin-embedded mouse ventricle tissue, as well as in Western blot on mouse liver (1:2000), where a clear band appeared at 35 kDa. One reviewer noted the antibody works well when diluted in 1× TBST. Overall, users found the staining results strong and the recommended dilutions effective across applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kamal Baral (Verified Customer) (01-26-2024) | Bone marrow mesenchymal stem cells from mouse stained great at the provided dilution.
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Kamal (Verified Customer) (01-26-2024) | Bands appeared at 35 kDa. Antibody works well when diluted in 1X TBST.
|
Alexandru (Verified Customer) (11-13-2023) | Great stainig results on paraffin-embedded tissue!
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