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CCR2a-specific Polyclonal antibody

CCR2a-specific Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 16153-1-AP


C C chemokine receptor type 2, C C CKR 2, CC CKR 2, CCR 2, CCR2, CCR2a, CCR2a-specific, CCR2b, CD192, CKR2, CKR2A, CKR2B, CMKBR2, MCP 1 R

Tested Applications

Positive WB detected inJurkat cells, mouse skeletal muscle tissue, rat skeletal muscle tissue
Positive IHC detected inmouse kidney tissue, human kidney tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16153-1-AP targets CCR2a-specific in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name chemokine (C-C motif) receptor 2
Calculated Molecular Weight 42 kDa
Observed Molecular Weight35 kDa
GenBank Accession NumberNM_000647
Gene Symbol CCR2
Gene ID (NCBI) 729230
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration, and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions, and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.


Product Specific Protocols
WB protocol for CCR2a-specific antibody 16153-1-APDownload protocol
IHC protocol for CCR2a-specific antibody 16153-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Lab Invest

Matrix metalloproteinase 11 (MMP11) in macrophages promotes the migration of HER2-positive breast cancer cells and monocyte recruitment through CCL2-CCR2 signaling.

Authors - Shin Ung Kang

Oncol Rep

CCL2/CCR2 axis induces hepatocellular carcinoma invasion and epithelial-mesenchymal transition in vitro through activation of the Hedgehog pathway.

Authors - Huijie Zhuang

J Ethnopharmacol

Identification of potential regulating effect of baicalin on NFκB/CCL2/CCR2 signaling pathway in rats with cerebral ischemia by antibody-based array and bioinformatics analysis

Authors - Tian Xu

Mol Immunol

QKI deficiency in macrophages protects mice against JEV infection by regulating cell migration and antiviral response.

Authors - Lele Deng

J Physiol Biochem

Hypoxia-induced CCL2/CCR2 axis in adipose-derived stem cells (ADSCs) promotes angiogenesis by human dermal microvascular endothelial cells (HDMECs) in flap tissues

Authors - Bairong Fang

Clin Exp Rheumatol

Curcumin alleviates inflammation in Takayasu's arteritis by blocking CCL2 overexpression in adventitial fibroblasts.

Authors - Sifan Wu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kamal Baral (Verified Customer) (01-26-2024)

Bone marrow mesenchymal stem cells from mouse stained great at the provided dilution.

  • Applications: Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse bone marrow

Kamal (Verified Customer) (01-26-2024)

Bands appeared at 35 kDa. Antibody works well when diluted in 1X TBST.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse liver tissue

Alexandru (Verified Customer) (11-13-2023)

Great stainig results on paraffin-embedded tissue!

  • Applications: Immunofluorescence
  • Cell Tissue Type: Mouse ventricle