CCR2a-specific Polyclonal antibody
CCR2a-specific Polyclonal Antibody for FC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
human and More (2)
WB, IHC, IF, FC, ELISA
Cat no : 16153-1-AP
Validation Data Gallery
|Positive WB detected in||Jurkat cells, A431 cells|
|Positive IHC detected in||human kidney tissue, human ovary tumor tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
|WB||See 9 publications below|
|IHC||See 5 publications below|
|IF||See 2 publications below|
16153-1-AP targets CCR2a-specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||chemokine (C-C motif) receptor 2|
|Calculated molecular weight||42 kDa|
|Observed molecular weight||42 kDa|
|GenBank accession number||NM_000647|
|Gene ID (NCBI)||729230|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CCR2 encodes a receptor for MCP1, functioning in inflammatory processes, tumor infiltration and monocyte invasion of artery walls in atherosclerosis. There are two isoforms of CCR2, CCR2A and CCR2B. CCR2 isoforms were expressed by different cell subsets in both normal and IIM muscle. CCR2A was expressed in vessel walls and by some mononuclear cells, especially in cells involved in partial invasion in PM and IBM. CCR2B expression was observed in all satellite cells, in the muscular domain of neuromuscular junctions and in some regenerative fibers of IIM, but not in inflammatory exudates. The sequences of the two isoforms are different from 314-374 aa. This antibody 16153-1-AP is raised against the peptide of 344-358 amino acids of CCR2a. It is specific to CCR2a.
|Product Specific Protocols|
|WB protocol for CCR2a-specific antibody 16153-1-AP||Download protocol|
|IHC protocol for CCR2a-specific antibody 16153-1-AP||Download protocol|
|FC protocol for CCR2a-specific antibody 16153-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Physiol Biochem
Hypoxia-induced CCL2/CCR2 axis in adipose-derived stem cells (ADSCs) promotes angiogenesis by human dermal microvascular endothelial cells (HDMECs) in flap tissues
Matrix metalloproteinase 11 (MMP11) in macrophages promotes the migration of HER2-positive breast cancer cells and monocyte recruitment through CCL2-CCR2 signaling.
Identification of potential regulating effect of baicalin on NFκB/CCL2/CCR2 signaling pathway in rats with cerebral ischemia by antibody-based array and bioinformatics analysis
QKI deficiency in macrophages protects mice against JEV infection by regulating cell migration and antiviral response.
Clin Exp Rheumatol
Curcumin alleviates inflammation in Takayasu's arteritis by blocking CCL2 overexpression in adventitial fibroblasts.
CCL2-CCR2 Axis Potentiates NMDA Receptor Signaling to Aggravate Neuropathic Pain Induced by Brachial Plexus Avulsion.