Anti-Mouse CD14 Rabbit Recombinant Antibody

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  IF Staining of RAW 264.7 using 98020-1-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD14 antibody (98020-1-RR, Clone: 240225C2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of mouse peritoneal macrophages using 98020-1-RR

  1x10^6 mouse peritoneal macrophages were surface stained with 0.25 ug Anti-Mouse CD14 Rabbit Recombinant Antibody (98020-1-RR, Clone: 240225C2) and PE-conjugated Goat Anti-Rabbit IgG (red), or 0.25 ug Isotype Control and PE-conjugated Goat Anti-Rabbit IgG (blue). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98020-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98020-1-RR against Mouse CD14 were performed. The affinity constant is 0.383 nM.

CD14 Uni-rAb^TM Recombinant Antibody for IF/ICC, FC

Cat No. 98020-1-RR

Clone No.240225C2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, FC

Synonyms

CD14 antigen, 240225C2

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Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells
• Positive FC detected in: mouse peritoneal macrophages

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98020-1-RR targets CD14 in IF/ICC, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD14 antigen
• Calculated Molecular Weight: 39 kDa
• GenBank Accession Number: NM_009841.4
• Gene Symbol: Cd14
• Gene ID (NCBI): 12475
• RRID: AB_3672164
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P10810
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.