Validation Data Gallery
|Positive WB detected in||HepG2 cells, HeLa cells, A375 cells, L02 cells, HUVEC cells, human placenta tissue|
|Positive IHC detected in||human liver cancer tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue, HUVEC cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66153-1-Ig is CD146/MCAM Fusion Protein expressed in E. coli.
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD146/MCAM fusion protein Ag11855|
|Full Name||melanoma cell adhesion molecule|
|Calculated molecular weight||646 aa, 72 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC056418|
|Gene ID (NCBI)||4162|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.
|Product Specific Protocols|
|WB protocol for CD146/MCAM antibody 66153-1-Ig||Download protocol|
|IHC protocol for CD146/MCAM antibody 66153-1-Ig||Download protocol|
|IF protocol for CD146/MCAM antibody 66153-1-Ig||Download protocol|
|FC protocol for CD146/MCAM antibody 66153-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (02-05-2021)
Not sure whether it works, because I cannot detect anything in whole RPE1 cell lysates.