CD146/MCAM Monoclonal antibody
CD146/MCAM Monoclonal Antibody for FC, IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
WB, IHC, IF, FC, ELISA
Cat no : 66153-1-Ig
Validation Data Gallery
|Positive WB detected in||HepG2 cells, HeLa cells, A375 cells, L02 cells, HUVEC cells, human placenta tissue|
|Positive IHC detected in||human liver cancer tissue, human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue, HUVEC cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunohistochemistry (IHC)||IHC : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:1000-1:4000|
|Sample-dependent, check data in validation data gallery|
The immunogen of 66153-1-Ig is CD146/MCAM Fusion Protein expressed in E. coli.
|Host / Isotype||Mouse / IgG1|
|Immunogen||CD146/MCAM fusion protein Ag11855|
|Full Name||melanoma cell adhesion molecule|
|Calculated molecular weight||646 aa, 72 kDa|
|Observed molecular weight||120 kDa|
|GenBank accession number||BC056418|
|Gene ID (NCBI)||4162|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.
|Product Specific Protocols|
|WB protocol for CD146/MCAM antibody 66153-1-Ig||Download protocol|
|IHC protocol for CD146/MCAM antibody 66153-1-Ig||Download protocol|
|IF protocol for CD146/MCAM antibody 66153-1-Ig||Download protocol|
|FC protocol for CD146/MCAM antibody 66153-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Exp Cell Res
Nrf2 activation is involved in osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (02-05-2021)
Not sure whether it works, because I cannot detect anything in whole RPE1 cell lysates.