CD146/MCAM Monoclonal antibody

CD146/MCAM Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1









Cat no : 66153-1-Ig


CD146, MCAM, MUC18

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, A375 cells, L02 cells, HUVEC cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue, HUVEC cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66153-1-Ig targets CD146/MCAM in WB, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD146/MCAM fusion protein Ag11855
Full Name melanoma cell adhesion molecule
Calculated Molecular Weight 646 aa, 72 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC056418
Gene Symbol MCAM
Gene ID (NCBI) 4162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.


Product Specific Protocols
WB protocol for CD146/MCAM antibody 66153-1-IgDownload protocol
IHC protocol for CD146/MCAM antibody 66153-1-IgDownload protocol
IF protocol for CD146/MCAM antibody 66153-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Boyan (Verified Customer) (02-05-2021)

Not sure whether it works, because I cannot detect anything in whole RPE1 cell lysates.

  • Applications: Western Blot