PE-conjugated CD146/MCAM Monoclonal antibody

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  IF Staining of human colon cancer using PE-66153

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using PE CD146/MCAM antibody (PE-66153, Clone: 4D8A9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using PE-66153

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using PE CD146/MCAM antibody (PE-66153, Clone: 4D8A9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD146/MCAM Monoclonal Antibody for IF-P

Cat No. PE-66153

Clone No.4D8A9

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF-P

Synonyms

CD146, 4D8A9, MCAM, Melanoma-associated antigen A32, Melanoma-associated antigen MUC18

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-66153 targets CD146/MCAM in IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD146/MCAM fusion protein Ag11855
• Full Name: melanoma cell adhesion molecule
• Calculated Molecular Weight: 646 aa, 72 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC056418
• Gene Symbol: CD146
• Gene ID (NCBI): 4162
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P43121
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988). This antibody detects a band at approximately 120 kDa that corresponds to the molecular weight of glycosylated CD146. Treatment of lysates of HepG2 cells and L02 cells with PNGase F, which removes oligosaccharides from N-linked glycoproteins, led to a down-shift of the detected band.