Anti-Rabbit CD19 Mouse Recombinant Antibody

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  FC experiment of rabbit splenocytes using 98351-1-MR

  1x10^6 rabbit splenocytes were surface stained with 0.25 ug Anti-Rabbit CD19 Mouse RecAb (98351-1-MR, Clone:242570A11) .or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4)and PE-conjugated Goat Anti-Mouse IgG. Cells were then stained with Anti-Rabbit FITC-CD4. Cells were not fixed.

• 

  FC experiment of rabbit splenocytes using 98351-1-MR

  1x10^6 rabbit splenocytes were surface stained with 0.25 ug Anti-Rabbit CD19 Mouse RecAb (98351-1-MR, Clone:242570A11)(red).or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4)and PE-conjugated Goat Anti-Mouse IgG(blue). Cells were not fixed.

• 

  FC experiment of rabbit splenocytes using 98351-1-MR

  1x10^6 rabbit splenocytes were surface stained with 0.25 ug Anti-Rabbit CD19 Mouse RecAb (98351-1-MR, Clone:242570A11).or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4)and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

CD19 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98351-1-MR

Clone No.242570A11

Host / Isotype

Mouse / IgG

Reactivity

rabbit

Applications

FC

Synonyms

CD19 molecule

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Tested Applications

• Positive FC detected in: rabbit splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98351-1-MR targets CD19 in FC applications and shows reactivity with rabbit samples.

• Tested Reactivity: rabbit
• Host / Isotype: Mouse / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD19 fusion protein Eg3526
• Full Name: CD19
• Gene Symbol: CD19
• Gene ID (NCBI): 100345233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).