Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65208-1-Ig targets IgG2a Isotype Control in FC, IP, CoIP applications and shows reactivity with samples.
| Tested Reactivity | |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | IgG2a Isotype Control |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2918470 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
The C1.18.4 immunoglobulin is useful as an isotype-matched control. The C.1.18.4 immunolglobulin has an unknown binding specificity and is used as an isotype control for mouse IgG2a antibodies. This antibody has been quality-tested for flow cytometry as negative control.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IgG2a Isotype Control antibody 65208-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Mouse IgG2a Isotype Control (C1.18.4), catalog number 65208-1-Ig, has 6 citations published in Nucleic Acids Research, International Journal of Biological Macromolecules, Molecular Medicine, Veterinary Pathology, Journal of Nutritional Science and Vitaminology, and Cells. Research fields span RNA metabolic labeling and translational control, B-cell immunology in fish, cigarette smoke-induced COPD, canine intestinal T-cell lymphoma, antioxidant effects on keratinocytes, and age-associated keratinocyte senescence. Applications cited include IP, CoIP, and flow cytometry.
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Simultaneous measurement of nascent transcriptome and translatome using 4-thiouridine metabolic RNA labeling and translating ribosome affinity purification | ||
Int J Biol Macromol Chinese yam polysaccharide induces the differentiation and natural antibody secretion of IgM(+) B cells to prevent Aeromonas hydrophila infection in grass carp. | ||
Mol Med Progesterone (P4) ameliorates cigarette smoke-induced chronic obstructive pulmonary disease (COPD) | ||
Vet Pathol Comprehensive phenotypic and clonality analysis of intraepithelial lymphocytes of intestinal T-cell lymphoma and chronic enteropathy in dogs. | ||
J Nutr Sci Vitaminol (Tokyo) Cooperation of Cysteine, Ascorbic Acid, Pyridoxine, and α-Lipoic Acid in Antioxidant Effects on Human Keratinocytes and 3D Human Skin Models | ||
Cells Age-Associated YBX1 Phosphorylation Regulates the Keratinocyte Senescence-Associated Secretory Phenotype Through Translational Control. |
