CD22 Recombinant monoclonal antibody, PBS Only (Detector)

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  WB analysis using 84265-2-RR (same clone as 84265-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84265-2-RR (CD22 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84265-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01173-3

  Sandwich ELISA standard curve of MP01173-3, Human CD22 Recombinant Matched Antibody Pair - PBS only. 84265-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02324. 84265-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP01173-3

  The mean CD22 concentration was determined to be 445.39 ng/mL in Raji cell extract based on a 3.00 mg/mL extract load and 185.59 ng/mL in Ramos cell extract based on a 1.40 mg/mL extract load.

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  Sample test of MP01173-3

  Serum of six individual healthy human donors was measured. The CD22 concentration of detected samples was determined to be 0.36 ng/mL with a range of 0.25-0.54 ng/mL.

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  Cytometric bead array standard curve of MP01173-3

  Cytometric bead array standard curve of MP01173-3, CD22 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84265-4-PBS. Detection antibody: 84265-2-PBS. Standard: RP02324. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 84265-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84265-2-RR against Human CD22 were performed. The affinity constant is 61.3 pM.

CD22 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84265-2-PBS

Clone No.241314G10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241314G10, B cell receptor CD22, B-cell receptor CD22, BL-CAM, B-lymphocyte cell adhesion molecule

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Product Information

84265-2-PBS targets CD22 as part of a matched antibody pair:

MP01173-3: 84265-4-PBS capture and 84265-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: CD22 molecule
• Calculated Molecular Weight: 847 aa, 95 kDa
• Observed Molecular Weight: 130-140 kDa
• GenBank Accession Number: BC109306
• Gene Symbol: CD22
• Gene ID (NCBI): 933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P20273
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD22, also known as Siglec-2 (sialic acid binding Ig-like lectin 2) or BL-CAM (B-lymphocyte cell adhesion molecule), is a 130-140 kDa, B-cell restricted, type I transmembrane glycoprotein belonging to the immunoglobulin gene superfamily. The expression of CD22 is developmentally regulated. It is expressed at low levels in the cytoplasm of pro-B and pre-B cells and present on the cell surface only at mature stages of B-cell differentiation. Cell surface expression is lost during terminal differentiation into plasma cell and after B-cell activation. CD22 is an inhibitory receptor for B-cell receptor (BCR) signalling, preferentially binds to alpha-2,6-linked sialic acid and mediates B-cell B-cell interactions. It plays a crucial role in activation and differentiation of the B-cell.