PE Anti-Mouse CD25/IL-2RA (PC61.5)

• 

  FC experiment of mouse splenocytes using PE-65137

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with APC Anti-Mouse CD4 and 0.1 ug PE Anti-Mouse CD25/IL-2RA (PC61.5) (PE-65137, Clone: PC61.5). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using PE-65137

  1x10^6 anti-CD3/CD28-treated mouse splenocytes were surface stained with 0.1 ug PE Anti-Mouse CD25/IL-2RA (PC61.5) (PE-65137, Clone: PC61.5) (red) or PE Rat IgG1 Isotype Control (HRPN) (PE-65212, Clone: HRPN) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.1 ug PE Anti-Mouse CD25/IL-2RA (PC61.5) (PE-65137, Clone: PC61.5) (black, dashed). Cells were not fixed.

CD25/IL-2RA Monoclonal Antibody for FC

Cat No. PE-65137

Clone No.PC61.5

Host / Isotype

Rat / IgG1, lambda

Reactivity

mouse

Applications

FC

Synonyms

CD25, IL-2 receptor subunit alpha, Il2r, IL-2R subunit alpha, Il2ra

---

Tested Applications

• Positive FC detected in: Anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.1 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-65137 targets CD25/IL-2RA in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG1, lambda
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: interleukin 2 receptor, alpha chain
• GenBank Accession Number: BC114437
• Gene Symbol: CD25
• Gene ID (NCBI): 16184
• RRID: AB_2883919
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P01590
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).