FITC Plus Anti-Mouse CD25/IL-2RA (PC61.5)

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  FC experiment of mouse splenocytes using FITC-65137

  1x10^6 untreated or anti-CD3/CD28 treated (2 days) mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 and 0.25 ug FITC Plus Anti-Mouse CD25 (PC61.5) (FITC-65137, Clone: PC61.5). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using FITC-65137

  1x10^6 anti-CD3/CD28 treated (2 days) mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD25 (PC61.5) (FITC-65137, Clone: PC61.5) (red) or 0.25 ug FITC Plus Rat IgG1 Isotype Control (HRPN) (FITC-65212, Clone: HRPN) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD25 (PC61.5) (FITC-65137, Clone: PC61.5) (black, dashed). Cells were not fixed.

CD25/IL-2RA Monoclonal Antibody for FC

Cat No. FITC-65137

Clone No.PC61.5

Publications(5)

Host / Isotype

Rat / IgG1, lambda

Reactivity

mouse

Applications

FC

Synonyms

CD25, Il-2ra, Il2ra, IL-2R subunit alpha, Il2r

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Tested Applications

• Positive FC detected in: anti-CD3/CD28 treated mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 3 publications below

Product Information

FITC-65137 targets CD25/IL-2RA in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG1, lambda
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  N/A

• Full Name: interleukin 2 receptor, alpha chain
• GenBank Accession Number: BC114437
• Gene Symbol: CD25
• Gene ID (NCBI): 16184
• RRID: AB_2883797
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P01590
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	FC	Oncogene; ZG16 enhances the maturation of dendritic cells via induction of CD40 and contributes to the antitumor immunity in pancreatic cancer; Authors - Hui Meng
mouse	FC	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
		Cell Rep; Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses; Authors - Bin Qian
Mouse		BMC Pharmacol Toxicol; Blocking group 2 innate lymphoid cell activation and macrophage M2 polarization: potential therapeutic mechanisms in ovalbumin-induced allergic asthma by calycosin; Authors - Chunyan Tian
mouse	FC	J Colloid Interface Sci; Nano co-inducer of immunogenic cell death and ferroptosis for anti-tumor immunotherapy; Authors - Guanhong Guo