FITC Plus Anti-Mouse CD25 (PC61.5)

CD25 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG1, lambda

Reactivity

Mouse and More (1)

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

Publications

3

CloneNo.

PC61.5

Cat no : FITC-65137

Synonyms

CD25, Il2r, Il2ra, Il-2ra, Ly 43

---

Validation Data Gallery

• 

  FC experiment of mouse splenocytes using FITC-65137

  1X10^6 mouse splenocytes were surface stained with CoraLite® Plus 647 Anti-Mouse CD4 (GK1.5) (CL647-65104, Clone: GK1.5) and 0.13 ug FITC Plus Rat IgG1 isotype control (left) or 0.13 ug FITC Plus Anti-Mouse CD25 (FITC-65137, Clone: PC61.5) (right). Cells were not fixed.

---

Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• FC: See 2 publications below

Product Information

FITC-65137 targets CD25 in FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG1, lambda
• Class: Monoclonal
• Type: Antibody
• Immunogen: N/A
• Full Name: interleukin 2 receptor, alpha chain
• GenBank Accession Number: BC114437
• Gene Symbol: CD25
• Gene ID (NCBI): 16184
• RRID: AB_2883797
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+Foxp3+ Treg cells (PMID: 22585674).

Publications

Species	Application	Title
	FC	Oncogene; ZG16 enhances the maturation of dendritic cells via induction of CD40 and contributes to the antitumor immunity in pancreatic cancer; Authors - Hui Meng
mouse	FC	Phytomedicine; Multi-material basis and multi-mechanisms of the Dahuang Zhechong pill for regulating Treg/Th1 balance in hepatocellular carcinoma.; Authors - Li Wu
		Cell Rep; Podocyte SIRPα reduction aggravates lupus nephritis via promoting T cell inflammatory responses; Authors - Bin Qian