CD25/IL-2R alpha Recombinant antibody

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  WB analysis using 86213-1-RR

  Recombinant protein were subjected to SDS PAGE followed by western blot with 86213-1-RR (CD25/IL-2R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86213-1-RR

  CTLL-2 cells were subjected to SDS PAGE followed by western blot with 86213-1-RR (CD25/IL-2R alpha antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

CD25/IL-2R alpha Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86213-1-RR

Clone No.250670H3

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, ELISA

Synonyms

CD25, IL-2 receptor subunit alpha, IL-2R subunit alpha, Il2ra, IL-2-RA

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Tested Applications

• Positive WB detected in: CTLL-2 cells, Recombinant protein

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86213-1-RR targets CD25/IL-2R alpha in WB, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD25/IL-2R alpha fusion protein Eg1453
• Full Name: interleukin 2 receptor, alpha
• Calculated Molecular Weight: 31kDa
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: NM_013163.1
• Gene Symbol: Il2ra
• Gene ID (NCBI): 25704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P26897
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674).