CoraLite® Plus 647 Anti-Human CD300e Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using CL647-98235

  1x10^6 human PBMCs were surface stained with FITC Plus Anti-Human CD14 Rabbit Recombinant Antibody (FITC-98040, Clone: 230332D7), and 0.25 ug CoraLite® Plus 647 Anti-Human CD300e Rabbit RecAb (CL647-98235, Clone:242031C10) or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9). Cells were not fixed.

CD300e Uni-rAb^TM Recombinant Antibody for FC

Cat No. CL647-98235

Clone No.242031C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

242031C10, CD300LE, CLM 2, CLM2, CMRF35 A5

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-98235 targets CD300e in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD300e fusion protein Eg2638
• Full Name: CD300e molecule
• Calculated Molecular Weight: 23 kDa
• GenBank Accession Number: NM_181449.2
• Gene Symbol: CD300E
• Gene ID (NCBI): 342510
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q496F6
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD300e, also known as immune receptor expressed on myeloid cells 2 (IREM-2), CLM-2, or CMRF35-A5, is one of the CD300 family members which belong to the immunoglobulin (Ig) superfamily (PMID: 15557162; 20039296). CD300e is a type I transmembrane glycoprotein that contains an extracellular region comprising an Ig-like domain, a transmembrane region, and a short cytoplasmic tail (PMID: 15557162; 29358324). It is by monocytes and myeloid dendritic cells and transmits an immune-activating signal by interacting with DNAX-activating protein 12 (DAP12) (PMID: 29358324).