CD34 Monoclonal antibody

CD34 Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG2b









Cat no : 60180-1-Ig


CD34, CD34 molecule

Tested Applications

Positive WB detected inhuman placenta tissue, human uterus tissue, human testis tissue
Positive IHC detected inhuman liver cancer tissue, human placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman placenta tissue, human liver cancer tissue, human tonsillitis tissue
Positive FC detected inHUVEC cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60180-1-Ig targets CD34 in WB, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD34 fusion protein Ag5996
Full Name CD34 molecule
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 105 kDa
GenBank Accession NumberBC039146
Gene Symbol CD34
Gene ID (NCBI) 947
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).


Product Specific Protocols
WB protocol for CD34 antibody 60180-1-IgDownload protocol
IHC protocol for CD34 antibody 60180-1-IgDownload protocol
IF protocol for CD34 antibody 60180-1-IgDownload protocol
FC protocol for CD34 antibody 60180-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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