CD34 Monoclonal antibody

CD34 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF, FC

Conjugate

Unconjugated

CloneNo.

3C8G12

Cat no : 60180-1-Ig

Synonyms

CD34, CD34 molecule



Tested Applications

Positive WB detected inhuman placenta tissue, human uterus tissue, human testis tissue
Positive IHC detected inhuman liver cancer tissue, human placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue, human liver cancer tissue
Positive FC detected inHUVEC cells
Planning an IHC experiment? We recommend our IHCeasy CD34 Ready-To-Use IHC Kit. CD34 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

60180-1-Ig targets CD34 in WB, IHC, IF, FC applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CD34 fusion protein Ag5996
Full Name CD34 molecule
Calculated molecular weight 41 kDa
Observed molecular weight 105 kDa
GenBank accession numberBC039146
Gene symbol CD34
Gene ID (NCBI) 947
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Protocols

Product Specific Protocols
WB protocol for CD34 antibody 60180-1-IgDownload protocol
IHC protocol for CD34 antibody 60180-1-IgDownload protocol
IF protocol for CD34 antibody 60180-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Dose Response

Low-Dose Radiation Enhanced Inhibition of Breast Tumor Xenograft and Reduced Myocardial Injury Induced by Doxorubicin.

Authors - Xin Jiang
humanIHC

J Exp Clin Cancer Res

ZRANB2/SNHG20/FOXK1 Axis regulates Vasculogenic mimicry formation in glioma.

Authors - Xiaozhi Li
humanIF

Arthritis Res Ther

Divergent roles of prostacyclin and PGE2 in human tendinopathy.

Authors - Filip Bergqvist
mouseIF

J Neuroinflammation

Meningitic Escherichia coli-induced upregulation of PDGF-B and ICAM-1 aggravates blood-brain barrier disruption and neuroinflammatory response.

Authors - Rui-Cheng Yang
humanIF

Oncotarget

Induction of VEGFA and Snail-1 by meningitic Escherichia coli mediates disruption of the blood-brain barrier.

Authors - Ruicheng Yang
humanIHC

Stem Cells Int

Biocompatibility and Angiogenic Effect of Chitosan/Graphene Oxide Hydrogel Scaffolds on EPCs.

Authors - Lifang Zhang