Validation Data Gallery
|Positive WB detected in||human placenta tissue, human uterus tissue, human testis tissue|
|Positive IHC detected in||human liver cancer tissue, human placenta tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human tonsillitis tissue, human liver cancer tissue|
|Positive FC detected in||HUVEC cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:2500-1:10000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
60180-1-Ig targets CD34 in WB, IHC, IF, FC applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2b|
|Immunogen||CD34 fusion protein Ag5996|
|Full Name||CD34 molecule|
|Calculated molecular weight||41 kDa|
|Observed molecular weight||105 kDa|
|GenBank accession number||BC039146|
|Gene ID (NCBI)||947|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.
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