CD34 Monoclonal antibody, PBS Only

CD34 Monoclonal Antibody for WB, IF, FC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

CloneNo.

3C8G12

Cat no : 60180-1-PBS

Synonyms

CD34, CD34 molecule

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Validation Data Gallery

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  WB analysis of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 60180-1-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human uterus using 60180-1-Ig (same clone as 60180-1-PBS)

  human uterus tissue were subjected to SDS PAGE followed by western blot with 60180-1-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human testis using 60180-1-Ig (same clone as 60180-1-PBS)

  human testis tissue were subjected to SDS PAGE followed by western blot with 60180-1-Ig (CD34 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60180-1-Ig (CD34 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60180-1-Ig (CD34 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 60180-1-Ig (CD34 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human liver cancer using 60180-1-Ig (same clone as 60180-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD34 antibody (60180-1-Ig, Clone: 3C8G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HUVEC using 60180-1-Ig (same clone as 60180-1-PBS)

  1X10^6 HUVEC cells were stained with 0.20 ug Anti-Human CD34 (60180-1-Ig, Clone:3C8G12) (red) or 0.20 ug control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution of 1:1000. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 60180-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60180-1-PBS targets CD34 in WB, IF, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD34 fusion protein Ag5996
• Full Name: CD34 molecule
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC039146
• Gene Symbol: CD34
• Gene ID (NCBI): 947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.