Anti-Mouse CD41 (MWReg30)

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  FC experiment of C57BL/6 mouse peripheral blood platelets using 65189-1-Ig

  1x10^6 C57BL/6 mouse peripheral blood platelets were surface stained with 0.5 ug Anti-Mouse CD41 (65189-1-Ig, Clone: MWReg30) and FITC anti-rat IgG1 Antibody (red), or 0.5 ug Rat IgG1 Isotype Control (HRPN) (65212-1-Ig, Clone: HRPN) and FITC anti-rat IgG1 Antibody (blue). Cells were not fixed.

CD41 Monoclonal Antibody for FC

Cat No. 65189-1-Ig

Clone No.MWReg30

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Synonyms

Itga2b, GpIIb, integrin alpha 2b, MWReg30

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Tested Applications

• Positive FC detected in: C57BL/6 mouse peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

65189-1-Ig targets CD41 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse platelets
• Full Name: integrin alpha 2b
• GenBank Accession Number: BC120493
• Gene Symbol: CD41
• Gene ID (NCBI): 16399
• RRID: AB_3084902
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Purified by protein-G affinity chromatography
• UNIPROT ID: Q9QUM0
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID: 19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light beta (25 kDa) and heavy alpha (125 kDa) chains, which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. CD41/CD61 is crucial for platelet aggregation through binding of soluble fibrinogen.