CoraLite® Plus 488 Anti-Mouse CD41 (MWReg30)

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  FC experiment of Balb/c mouse peripheral blood platelets using CL488-65189

  1X10^6 Balb/c mouse peripheral blood platelets were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD41 (CL488-65189, Clone: MWReg30) (red), or 0.5 ug Isotype Control. Cells were not fixed.

• 

  FC experiment of Balb/c mouse peripheral blood platelets using CL488-65189

  1X10^6 Balb/c mouse peripheral blood platelets were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD41 (CL488-65189, Clone: MWReg30), or 0.5 ug Isotype Control. Cells were not fixed.

CD41 Monoclonal Antibody for FC

Cat No. CL488-65189

Clone No.MWReg30

Host / Isotype

Rat / IgG1, kappa

Reactivity

mouse

Applications

FC

Synonyms

Itga2b, GpIIb, integrin alpha 2b, MWReg30

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Tested Applications

• Positive FC detected in: mouse peripheral blood platelets

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-65189 targets CD41 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rat / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse platelets
• Full Name: integrin alpha 2b
• GenBank Accession Number: BC120493
• Gene Symbol: CD41
• Gene ID (NCBI): 16399
• RRID: AB_3084162
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: Q9QUM0
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID: 19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light beta (25 kDa) and heavy alpha (125 kDa) chains, which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. CD41/CD61 is crucial for platelet aggregation through binding of soluble fibrinogen.