FITC Plus Anti-Mouse CD48 (HM48-1)

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  FC experiment of mouse splenocytes using FITC-65227

  1x10^6 mouse splenocytes were surface stained with 0.25 ug FITC Plus Anti-Mouse CD48 (HM48-1) (FITC-65227, Clone: HM48-1) (red) or FITC Plus Armenian Hamster IgG Isotype Control (PIP) (FITC-65210, Clone: PIP) (blue). Cells were not fixed.

CD48 Monoclonal Antibody for FC

Cat No. FITC-65227

Clone No.HM48-1

Host / Isotype

Armenian Hamster / IgG

Reactivity

mouse

Applications

FC

Synonyms

BLAST, BLAST 1, BLAST1, CD48 antigen, HM48-1

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-65227 targets CD48 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Armenian Hamster / IgG
• Class: Monoclonal
• Type: Antibody
• Immunogen: Mouse T lymphoma MBL-2
• Full Name: CD48 antigen
• GenBank Accession Number: BC060977
• Gene Symbol: Cd48
• Gene ID (NCBI): 12506
• RRID: AB_2924144
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P18181
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.