CD48 Recombinant antibody

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  WB analysis using 83871-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83871-1-RR (CD48 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of EL-4-B5 using 83871-1-RR

  Immunofluorescent analysis of (4% PFA) fixed EL-4-B5 cells using CD48 antibody (83871-1-RR, Clone: 240732E3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 83871-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83871-1-RR against Mouse CD48 were performed. The affinity constant is 0.629 nM.

CD48 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 83871-1-RR

Clone No.240732E3

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

MEM 102, CD48 antigen, BLAST1, BLAST 1, BLAST

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Tested Applications

• Positive WB detected in: CTLL-2 cells, EL-4 cells, mouse spleen tissue
• Positive IF/ICC detected in: EL-4-B5 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83871-1-RR targets CD48 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: CD48 antigen
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 45-60 kDa
• GenBank Accession Number: BAE96326.1
• Gene Symbol: Cd48
• Gene ID (NCBI): 12506
• RRID: AB_3671457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P18181
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Signaling lymphocytic activation molecule family 2 (SLAMF2, CD48) is an adhesion and costimulatory molecule expressed constitutively on most hematopoietic cells, particularly in antigen-presenting cells (APC). CD48 is expressed on all the hematopoietic cells, both in humans and mice, except for murine neutrophils and long-term HSC. CD48 exists as both a membrane-bound (mCD48) and a soluble (sCD48) form. CD48 can activate T cells, antigen-presenting cells and granulocytes, by binding to CD2 or bacterial FimH, and through cell intrinsic effects. Interactions between CD48 and its high-affinity ligand CD244 are more complex, with both stimulatory and inhibitory outcomes. CD48 expression is increased in autoimmunity and allergy diseases, and anti-CD48 monoclonal antibodies have been shown to attenuate experimental autoimmune encephalomyelitis.