PE Anti-Human CD49b (AK7)

• 

  FC experiment of human PBMCs using PE-65252

  1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD49b (PE-65252, Clone:AK7) or unstained. Cells were not fixed. Monocytes were gated.

• 

  FC experiment of human PBMCs using PE-65252

  1X10^6 human PBMCs were surface stained with 5 ul PE Anti-Human CD49b (PE-65252, Clone:AK7) or unstained. Cells were not fixed. Monocytes were gated.

CD49b Monoclonal Antibody for FC

Cat No. PE-65252

Clone No.AK7

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Synonyms

BR, CD49B, Collagen receptor, GPIa, Integrin alpha 2, ITGA2, VLA 2, VLA 2 subunit alpha, VLAA2

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in 100 μl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-65252 targets CD49b in FC applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Human platelets

• Full Name: integrin, alpha 2 (CD49B, alpha 2 subunit of VLA-2 receptor)
• Calculated Molecular Weight: 126 kDa
• GenBank Accession Number: NM_002203
• Gene Symbol: Integrin alpha 2
• Gene ID (NCBI): 3673
• RRID: AB_3085024
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: P17301
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

CD49b, also known as alpha2 integrin, is a type I transmembrane glycoprotein which forms an adhesion molecule VLA-2 with beta1 integrin. CD49b is a receptor for many (extracellular) matrix and non-matrix molecules (PMID: 23847623). CD49b is expressed on numerous cell types including platelets, activated T cells, B cells, and monocytes. CD49b activation skews NK cell effector function to a phenotype that exhibits elevated IFN-γ production with a concomitant inhibition of motility and cytolytic function (PMID: 32971468, 32762681). The Anti-Human CD49b antibody, clone AK7, is derived from Human platelets.