CD53 Recombinant antibody, PBS Only

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  WB analysis using 84837-1-RR (same clone as 84837-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84837-1-RR (CD53 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84837-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84837-1-RR (same clone as 84837-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84837-1-RR (CD53 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84837-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84837-1-RR (same clone as 84837-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84837-1-RR (CD53 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84837-1-PBS in a different storage buffer formulation.

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  IF Staining of Daudi using 84837-1-RR (same clone as 84837-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD53 antibody (84837-1-RR, Clone: 242038C5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84837-1-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 84837-1-RR (same clone as 84837-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD53 antibody (84837-1-RR, Clone: 242038C5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84837-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84837-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84837-1-RR against Human CD53 were performed. The affinity constant is below 1 pM.

CD53 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 84837-1-PBS

Clone No.242038C5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

242038C5, Cell surface glycoprotein CD53, Leukocyte surface antigen CD53, MOX44, Tetraspanin 25

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Product Information

84837-1-PBS targets CD53 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: CD53 fusion protein Eg2182
• Full Name: CD53 molecule
• Calculated Molecular Weight: 24kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: NM_000560.4
• Gene Symbol: CD53
• Gene ID (NCBI): 963
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P19397
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

CD53 is also named as MOX44 and Tetraspanin-25 (Tspan-25). Tetraspanin CD53 is a member of the tetraspanin superfamily expressed exclusively within the immune compartment (PMID: 32440787). T cells depend on the phosphatase CD45 to initiate T cell receptor signaling. CD53 is shown to stabilize CD45 on the membrane and is required for optimal phosphatase activity and subsequent Lck activation. Together, CD53 as a regulator of CD45 activity required for T cell immunity (PMID: 35767951). CD53 is a marker for positively selected CD4+ CD8+ thymocytes (PMID: 11867561).