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Recombinant Human CD53 protein (rFc Tag)

Species

Human

Purity

>90 %, SDS-PAGE

Tag

rFc Tag

Activity

not tested

Cat no : Eg2182

Validation Data Gallery

  • Purity of Recombinant Human CD53 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD53 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Product Information

Purity >90 %, SDS-PAGE
Endotoxin <0.1 EU/μg protein, LAL method
Activity 
Not tested
Expression HEK293-derived Human CD53 protein Glu107-Asn181 (Accession# P19397) with a rabbit IgG Fc tag at the C-terminus.
GeneID 963
Accession P19397
PredictedSize 34.6 kDa
SDS-PAGE 38-50 kDa, reducing (R) conditions
Formulation Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage Conditions
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

CD53 is a structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. It participates in diverse biological functions such as cell signal transduction, adhesion, migration, and protein trafficking. It also plays a role in the activation of monocytes and B-cells.

References:

1. Malou Zuidscherwoude, et al. (2017) Sci Signal. May 9;10(478):eaag2755. 
2. Yihu Yang, et al. (2020) EMBO J. Sep 15;39(18):e105246.
3. Vera-Marie E Dunlock, Abbey B Arp, et al. (2022) Cell Rep. Jun 28;39(13):111006.
4. J Olweus, F Lund-Johansen, V Horejsi. (1993) J Immunol. Jul 15;151(2):707-16.