CD55 Polyclonal antibody

• Featured Product
• KD/KO Validated

CD55 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 26580-1-AP

Synonyms

TC, DAF, CROM, CR, Complement decay-accelerating factor

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Validation Data Gallery

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  WB analysis of HeLa using 26580-1-AP

  WB result of CD55 antibody (26580-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD55 transfected HeLa cells.

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  WB analysis using 26580-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26580-1-AP (CD55 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 26580-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26580-1-AP (CD55 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 26580-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26580-1-AP (CD55 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26580-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26580-1-AP (CD55 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 26580-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26580-1-AP (CD55 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 26580-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CD55 antibody (26580-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, MKN-45 cells, human placenta tissue
• Positive IHC detected in: human placenta tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• FC: See 1 publications below

Product Information

26580-1-AP targets CD55 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CD55 fusion protein Ag25026
• Full Name: CD55 molecule, decay accelerating factor for complement (Cromer blood group)
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 60-80 kDa
• GenBank Accession Number: BC001288
• Gene Symbol: CD55
• Gene ID (NCBI): 1604
• RRID: AB_2880559
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD55, also known as DAF, is a glycosylphosphatidylinositol (GPI)-anchored surface glycoprotein that is widely distributed on blood, stroma, epithelial, and endothelial cells (PMID: 7517044; 29503741). It can also exist as a soluble form in plasma, urine, saliva, tears, and synovial fluids (PMID: 29503741). CD55 is a complement regulatory protein (PMID: 2469439; 7517044). It inhibits formation of the C3 convertases through binding to C3b and C4b. It also binds the alternate pathway convertase C3bBb, the classical pathway convertase and C4b2a to accelerate their decay (PMID: 17289551). CD55 also serves as a receptor for coxsackieviruses B1, B3, and B5 and several enteroviruses (PMID: 7538177; 7517044). The observed molecular weight of mature CD55 varies between 50 to 100 kDa depending on the cell type. Different sizes of CD55 might be caused by alternative splicing or different glycosylation patterns (PMID: 29503741).

Publications

Species	Application	Title
human	WB,IHC,IF	Mol Cancer; Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer; Authors - Rashmi Bharti
rat	WB	Nat Commun; Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model; Authors - Mengyan Hu
human	WB,FC	Clin Exp Immunol; Therapeutic hyperthermia regulates complement C3 activation and suppresses tumor development through HSPA5/NFκB/CD55 pathway in nasopharyngeal carcinoma; Authors - Chengcong Chen
rat	WB	Phytomedicine; Total coumarin derivates from Hydrangea paniculata attenuate renal injuries in cationized-BSA induced membranous nephropathy by inhibiting complement activation and interleukin 10-mediated interstitial fibrosis.; Authors - Weida Wang
	WB	J Proteomics; Quantitative proteomic analysis of scleras in guinea pig exposed to wavelength defocus.; Authors - Yingying Wen
human,rat	IF	Exp Ther Med; Paeoniflorin-6'O-benzene sulfonate suppresses fibroblast-like synoviocytes proliferation and migration in rheumatoid arthritis through regulating GRK2-Gβγ interaction; Authors - Yuwen Zhang