CD71 Monoclonal antibody

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  WB analysis of HeLa using 66180-1-Ig

  WB result of CD71 antibody (66180-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD71 transfected HeLa cells.

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  WB analysis of Raji using 66180-1-Ig

  Untreated and PNGase F-treated lysates of Raji cells and HeLa cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  WB analysis using 66180-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

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  WB analysis of TF-1 using 66180-1-Ig

  TF-1 cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 66180-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 66180-1-Ig (CD71 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 66180-1-Ig

  IP result of anti-CD71 (IP:66180-1-Ig, 5ug; Detection:66180-1-Ig 1:1000) with HeLa cells lysate 2400ug.

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  IHC staining of human placenta using 66180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 66180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:40000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66180-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66180-1-Ig (CD71 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human breast cancer using 66180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of MCF-7 using 66180-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of K-562 using 66180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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  IF Staining of K-562 using 66180-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CD71 antibody (66180-1-Ig, Clone: 3C11F11 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

• Featured Product
• KD/KO Validated

CD71 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Cat No. 66180-1-Ig

Clone No.3C11F11

Publications(29)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA, Blocking assay

Synonyms

TFR1, TFR, T9, sTfR, p90

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Tested Applications

• Positive WB detected in: U-251 cells, A549 cells, HeLa cells, Raji cells, TF-1 cells, U2OS cells, A431 cells, HepG2 cells, K-562 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human placenta tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue, MCF-7 cells
• Positive IF/ICC detected in: MCF-7 cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 24 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

66180-1-Ig targets CD71 in WB, IHC, IF/ICC, IF-P, IP, ELISA, Blocking assay applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: CD71 fusion protein Ag21612
• Full Name: transferrin receptor (p90, CD71)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC001188
• Gene Symbol: CD71
• Gene ID (NCBI): 7037
• ENSEMBL Gene ID: ENSG00000072274
• RRID: AB_2881575
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02786
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.

Publications

Species	Application	Title
human	IF	Chem Eng J; Functionalized biological metal–organic framework with nanosized coronal structure and hierarchical wrapping pattern for enhanced targeting therapy; Authors - Huafeng Wang
human	WB	Adv Sci (Weinh); Sirtuin1 Suppresses Calcium Oxalate Nephropathy via Inhibition of Renal Proximal Tubular Cell Ferroptosis Through PGC-1α-mediated Transcriptional Coactivation; Authors - Chen Duan
human	WB	Cell Death Dis; Super-enhancer-driven MLX mediates redox balance maintenance via SLC7A11 in osteosarcoma; Authors - Weitang Guo
human	WB	J Cell Biol; The deubiquitinase ZRANB1 is an E3 ubiquitin ligase for SLC7A11 and regulates ferroptotic resistance; Authors - Shan Huang
human	WB	Cell Biosci; Chemical screen in zebrafish lateral line identified compounds that ameliorate neomycin-induced ototoxicity by inhibiting ferroptosis pathway; Authors - Yipu Fan
human,mouse	WB	Free Radic Biol Med; Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity; Authors - Bin Leng