Validation Data Gallery
|Positive WB detected in||TF-1 cells, A549 cells, HeLa cells, HepG2 cells, K-562 cells, Raji cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human placenta tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human breast cancer tissue, HeLa cells|
|Positive FC detected in||Raji cells|
|Western Blot (WB)||WB : 1:2000-1:20000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66180-1-Ig targets CD71 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human samples.
|Host / Isotype||Mouse / IgG2a|
|Immunogen||CD71 fusion protein Ag21612|
|Full Name||transferrin receptor (p90, CD71)|
|Calculated molecular weight||85 kDa|
|Observed molecular weight||90 kDa|
|GenBank accession number||BC001188|
|Gene ID (NCBI)||7037|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
|Product Specific Protocols|
|WB protocol for CD71 antibody 66180-1-Ig||Download protocol|
|IHC protocol for CD71 antibody 66180-1-Ig||Download protocol|
|IF protocol for CD71 antibody 66180-1-Ig||Download protocol|
|IP protocol for CD71 antibody 66180-1-Ig||Download protocol|
|FC protocol for CD71 antibody 66180-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
SNX-3 mediates retromer-independent tubular endosomal recycling by opposing EEA-1-facilitated trafficking.
J Biol Chem
The transferrin receptor CD71 regulates type II CD38, revealing tight topological compartmentalization of intracellular cyclic ADP-ribose production.
J Biol Chem
Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation.