- Featured Product
- KD/KO Validated
CD71 Monoclonal antibody
CD71 Monoclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human and More (1)
Applications
WB, IP, IHC, IF, FC, Blocking assay, ELISA
Conjugate
Unconjugated
CloneNo.
3C11F11
Cat no : 66180-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | U-251 cells, Raji cells, A549 cells, TF-1 cells, HeLa cells, U2OS cells, A431 cells, HepG2 cells, K-562 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human placenta tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | human breast cancer tissue |
Positive FC detected in | Raji cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 9 publications below |
IF | See 3 publications below |
Product Information
66180-1-Ig targets CD71 in WB, IP, IHC, IF, FC, Blocking assay, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | CD71 fusion protein Ag21612 |
Full Name | transferrin receptor (p90, CD71) |
Calculated molecular weight | 85 kDa |
Observed molecular weight | 90 kDa |
GenBank accession number | BC001188 |
Gene symbol | TFRC |
Gene ID (NCBI) | 7037 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for CD71 antibody 66180-1-Ig | Download protocol |
IHC protocol for CD71 antibody 66180-1-Ig | Download protocol |
IF protocol for CD71 antibody 66180-1-Ig | Download protocol |
IP protocol for CD71 antibody 66180-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Chem Eng J Functionalized biological metal–organic framework with nanosized coronal structure and hierarchical wrapping pattern for enhanced targeting therapy | ||
Free Radic Biol Med Targeting the Na+/K+ ATPase DR-region with DR-Ab improves doxorubicin-induced cardiotoxicity | ||
Bioeng Transl Med Multistage targeting and dual inhibiting strategies based on bioengineered tumor matrix microenvironment-mediated protein nanocages for enhancing cancer biotherapy. | ||
J Biol Chem Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation. | ||
J Biol Chem The transferrin receptor CD71 regulates type II CD38, revealing tight topological compartmentalization of intracellular cyclic ADP-ribose production.
| ||
BMC Cardiovasc Disord Role of ferroptosis-related genes in coronary atherosclerosis and identification of key genes: integration of bioinformatics analysis and experimental validation |