CoraLite® Plus 488-conjugated CD71 Monoclonal antibody

• 

  IF Staining of K-562 using CL488-66180

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 CD71 antibody (CL488-66180, Clone: 3C11F11 ) at dilution of 1:200.

• 

  IF Staining of MCF-7 using CL488-66180

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 CD71 antibody (CL488-66180, Clone: 3C11F11 ) at dilution of 1:200.

• 

  IF Staining of K-562 using CL488-66180

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 CD71 antibody (CL488-66180, Clone: 3C11F11 ) at dilution of 1:200.

• Featured Product
• KD/KO Validated

CD71 Monoclonal Antibody for IF/ICC

Cat No. CL488-66180

Clone No.3C11F11

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Synonyms

3C11F11, p90, sTfR, T9, TFR

---

Tested Applications

• Positive IF/ICC detected in: K-562 cells, MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66180 targets CD71 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21612

  Product name: Recombinant human CD71 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 513-757 aa of BC001188

  Sequence: VKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDID

• Full Name: transferrin receptor (p90, CD71)
• Calculated Molecular Weight: 85 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC001188
• Gene Symbol: CD71
• Gene ID (NCBI): 7037
• ENSEMBL Gene ID: ENSG00000072274
• RRID: AB_3084217
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02786
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CD71, also known as transferrin receptor protein 1 (TfR1), is a transmembrane glycoprotein composed of two disulfide-linked monomers, each of 90 kDa molecular weight. Each monomer binds one holo-transferrin molecule creating an iron-Tf-TfR complex which enters the cell by endocytosis. CD71 is present on actively proliferating cells and is essential for iron transport into proliferating cells.