Anti-Mouse CD84 Rabbit Recombinant Antibody

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  FC experiment of mouse splenocytes using 98076-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD84/SLAMF5 Rabbit Recombinant Antibody (98076-1-RR, Clone:240903B8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L), or Isotype Control. Cells were then stained with CoraLite® Plus 647 Anti-Mouse CD4. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98076-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD84/SLAMF5 Rabbit Recombinant Antibody (98076-1-RR, Clone:240903B8) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or Isotype Control(blue). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98076-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98076-1-RR against Mouse CD84/SLAMF5 were performed. The affinity constant is 0.297 nM.

CD84 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98076-1-RR

Clone No.240903B8

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD84/SLAMF5, SLAMF5, CD84 antigen, 240903B8

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98076-1-RR targets CD84 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: CD84 antigen
• Calculated Molecular Weight: 37 kDa
• Gene Symbol: Cd84
• Gene ID (NCBI): 12523
• RRID: AB_3672223
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q18PI6-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD84, also known as SLAMF5, is a member of the CD2 subfamily of the immunoglobulin receptor superfamily (PMID: 9310491). CD84 is a single-chain type-I glycoprotein composed of two extracellular Ig-like domains, a hydrophobic transmembrane region, and a cytoplasmic domain. It is broadly expressed on almost all leukocyte subsets. CD84 functions as a homophilic adhesion molecule, whose signaling can activate or inhibit leukocyte function depending on the cell type and its stage of activation or differentiation (PMID: 30522694).